Phytochemical screening, GC-MS analysis and in vitro antioxidant activity of pollen of Centella asiatica [Linn] urban a traditional medicinal plant

In the present study the crude extracts of pollen of Centella asiatica [Linn.] Urban were explored for their antioxidant potential using Ferric Reducing Power, Metal Chelating Activity and Trolox Equivalent Antioxidant Capacity assays. In crude extracts of pollen antioxidant components were initially extracted in methanol and further fractionated in solvents of different polarity, such as n-Hexane, Chloroform, Ethyl Acetate and Water exhibited reasonable antioxidant activity. The extract was found to contain large amounts of phenolic and flavonoid contents ranged from 143-1155 mg/1 of gallic acid equivalent [GAE] and 911-2488 mg/1 of quercetin [QE] respectively. Moreover, Super oxide Anion Radical Scavenging Activity and GS-MS analysis were also carried out

In vitro antioxidant potential and free radical scavenging activity of various extracts of pollen of Typha domigensis Pers

Antioxidant potential of the pollen of Typha domigensis Pers. using Ferric Reducing ower, Metal Chelating Activity and Trolox Equivalent Antioxidant Capacity [TEAC] assays has been carried out in the current research work. The antioxidant components were initially extracted from the pollen in methanol and were further fractionated in solvents of different polarity such as n-Hexane, Chloroform, Ethyl Acetate and Water. Methanol extract which was found to have high reducing power, total phenolic contents with high metal chelating activity, has considerable prospective to utilize as a natural antioxidant and be capable to link with the total phenolic contents of plant

Effects of antioxidants on the stability of soybean oil

Oils and fats when exposed to air or light and stored for a period of time, are oxidized and deteriorate to produce rancidity. Antioxidants are the compounds, which slow down or hinder the oxidation reaction. The present study was aimed to investigate the effects of different single antioxidant or a mixture of antioxidants on the storage of soybean oil. The single antioxidants used were TBHQ, BHT, BHA and mixture of BHT and BHA. Four samples of oil were stored for 15 weeks with the addition of antioxidants, and a control sample [without antioxidant] was run side by side. The determination was examined periodically, measuring refractive index, saponification value and unsaponifiable matter. The results indicate that synthetic antioxidants tested with soybean oil like TBHQ>BHT>BHT and BHA>BHA were ranked in decreasing order of effectiveness. There was a significant decline of peroxide value and FFA value of the oil with the addition of antioxidant as compared to the oil without any antioxidant. There was also found a slight change in the colour of the oil without antioxidant as compared to oil with antioxidant. No significant change was observed in the saponification value, iodine value and unsaponifiable matter with the addition of antioxidant