Unique morphology of intraoperative measurement of distal femur in northern malaysian females and its correlation with total knee arthroplasty implant sizing / Morfología única de la medición intraoperatoria del fémur distal en mujeres del norte de malasia y su correlación con el tamaño del implante en artroplastia total de rodilla

SUMMARY: Accurate implant sizing is one of the major determinants in defining the success of total knee arthroplasty (TKA). Current TKA implants are based on Caucasian knee morphometry which is well documented to be larger than Asian knees. Even more, with regards to sizing, implant mismatch tends to be more evident in the female population. This study was designed to evaluate the distal femur dimensions of Malaysian female patients who underwent TKA in our institution and to compare them with the current prosthetic system in use. A total of 199 female patients (207 knees) who underwent TKA were enrolled in this study. Intraoperatively, the AP dimensions (medial and lateral condyles) and mediolateral (ML) width were measured. Known dimension of the femoral component of the prosthetic knee system currently in use were compared with the morphological data. The average femoral component overhang was 2.11 mm (SD 3.94 mm). There was significant difference between the mean ML width of the resected femur and the femoral component (p<0.01). Analysis also revealed a significant positive and weak relationship between both, AP (medial and lateral) and ML dimension. The aspect ratio (ML/AP) of the native femur was generally smaller than the implant aspect ratio which is likely to cause overhang in our population. In general, this study shows that the knees of our female sub-population are even narrower than the other Asian female knees from previous studies. Implants should be designed based on the morphological data of the local population. Implant manufactures should tailor them to accommodate a smaller change in ML width for an increment in the AP length and provide several ML widths for one AP length to obtain a better fitting prosthesis hence curbing the problem of ML overhang.

RESUMEN: El tamaño exacto del implante es uno de los principales determinantes para definir el éxito de la artroplastía total de rodilla (ATR). Los implantes de TKA se basan en la morfometría de rodilla caucásica, cuyo tamaño está reportado como mayor que las rodillas asiáticas. Más aún, en lo que respecta al tamaño, el desajuste de los implantes tiende a ser más evidente en la población femenina. Este estudio fue diseñado para evaluar las dimensiones del fémur distal de pacientes mujeres malasias que se sometieron a ATR en nuestra institución y compararlas con el sistema protésico actual. En este estudio se incluyeron un total de 199 pacientes (207 rodillas) que se sometieron a ATR. Intraoperatoriamente, se midieron las dimensiones AP (cóndilos medial y lateral) y el ancho mediolateral (ML). Se comparó la dimensión conocida del componente femoral del sistema protésico de rodilla actualmente en uso con los datos morfológicos. El voladizo o sobresaliencia del componente femoral fue de 2,11 mm (DE 3,94 mm). Hubo una diferencia significativa entre el ancho ML medio del fémur resecado y el componente femoral (p <0,01). El análisis también reveló una relación significativa positiva y débil entre las dimensiones AP (medial y lateral) y ML. La relación de aspecto (ML / AP) del fémur nativo fue más pequeña que la relación de aspecto del implante, lo que probablemente cause un voladizo en nuestra población. En general, este estudio muestra que las rodillas de nuestra subpoblación femenina son incluso más estrechas que otras rodillas de mujeres asiáticas reportadas en estudios anteriores. Los implantes deben ser diseñados en base a los datos morfológicos de la población local. Los fabricantes de implantes deben adaptarlos a un cambio más pequeño en el ancho de ML para un incremento en la longitud AP y proporcionar varios anchos ML para una longitud AP con el objetivo de obtener una prótesis de mejor ajuste y frenar el problema del voladizo ML.

The effect of multisensory-adapted dental environment on children's behavior toward dental treatment A systematic review

Objective: The objective was to assess the effectiveness of multisensory-adapted environment on children's behavior toward dental treatment, specifically in special need children. Materials and Methods: The main health databases were selected such as SCOPUS, Medline, CINAHL, and Dentistry and Oral Sciences. A set of keywords was defined to identify the relevant article were (i.e., Snoezelen OR Multisensory OR sensory-adapted and Dentistry OR Oral). The articles were selected and extracted by two reviewers based on the Preferred Reporting Items for Systematic Review and Meta-analysis guidelines and some predetermined exclusion criteria. Furthermore, the risk of bias assessment was done. Results: A total of 317 papers were selected at the first phase from SCOPUS (97 papers), Medline (108 papers), CINAHL (110 papers), and Dentistry and Oral Sciences (2 papers). After applying the inclusion and exclusion criteria and duplicated papers were removed, only four eligible papers were selected for final synthesis. Conclusions: Multisensory-adapted dental environment effectively improves oral health behavior among special needs children in terms of physiological changes, behaviors, pain, and sensory discomfort. Thus, the clinician may introduce this approach in their clinical settings.

Growth, Body Composition and Resistance to Aeromonas hydrophila Challenge in Juvenile African Catfish (Clarias gariepinus) Fed Diets Supplemented with Spirulina (Arthrospira platensis)

This study evaluates the growth, body composition, and resistance to Aeromonas hydrophila challenge in juvenile African catfish (Clarias gariepinus) fed diets supplemented with spirulina (Arthrospira platensis). Five experimental diets containing different percentages of locally grown dried spirulina (0, 1, 3, 5 and 7%) were fed daily to catfish juveniles at 5% of their body weight. The growth and body composition of the catfish were determined up to 90 days of the study period. At day 91, the catfish were intraperitoneally injected with 107 CFU/ml of virulent A. hydrophila. Generally, the growth and body composition of the catfish in spirulina inclusion groups showed no significant difference with the control group. The survival rate following A. hydrophila challenge was significantly low in the control group compared to all of the other treatment groups. After the challenge trial, only the white blood cell count value was significantly higher in all of the groups supplemented with spirulina compared to the control group. We concluded that the locally grown spirulina do not improve growth and body composition, but it increased the catfish resistance towards A. hydrophila infection.

Formulation of Cost-effective Medium Using Urea as a Nitrogen Source for Arthrospira platensis Cultivation under Real Environment

Even though Arthrospira is a well-known superfood, it is not extensively commercialized in developing countries like Malaysia due to the high production cost with low biomass yield. Based on literature, the algal production cost can be discounted by reducing the cost of medium utilizing cheaper and readily available chemicals. Therefore, the present study was conducted to experiment the prospect of Arthrospira platensis cultivation in newly designed medium with commercial or industrial grade fertilizers under real environment. Consequently, growth and yield of A. platensis was investigated under outdoor condition using modified Kosaric medium (MKM) which was designed by substituting the major laboratory chemicals in standard Kosaric medium (SKM) with commercial grade baking soda, sea salt, urea, phosphoric acid, potassium hydroxide and Epsom salt. Urea as an alternative nitrogen resource to sodium nitrate was pulse-fed throughout the cultivation period. The algal growth was measured through optical density, biomass dry weight and chlorophyll a content. The algal yield was determined by calculating its productivity and specific growth rate. The growth and yield of A. platensis was significantly higher (p < 0.05) in MKM in terms of optical density with 2.541 ABS, biomass dry weight with 1.30 g L-1, chlorophyll a content with 12.96 mg L-1, productivity with 0.141 g L-1 d-1 and specific growth rate with 0.253 µ d-1 compared to SKM in eight days of cultivation period. The present finding showed the potential of MKM in lowering the medium cost up to 97% compared to SKM without compromising the algal yield under natural condition with proper cultivation techniques such as preadaptation and fed-batch addition of urea in the late evening.

Spectrophotometric Determination of Gentamicin Loaded PLGA Microparticles and Method Validation via Ninhydrin-Gentamicin Complex as a Rapid Quantification Approach.

The purpose of this study was to provide a quantification method with rapid, sensitive, reproducible and cost effective for gentamicin in the form of ninhydrin-gentamicin complex. The utilization of spectrophotometric module to validate the method development for gentamicin loaded microparticles was intended to provide alternative quantification method without undermining the sensitivity of the developed method. The microparticles fabrication process was proven to be suitable in encapsulating gentamicin by using poly(lactic-co-glycolic acid) PLGA without compromising the efficacy of the antibiotic itself. The linearity of 6 known concentrations of ninhydrin-gentamicin complex was obtained with the R2 of 0.9998. The interday and intraday precisions were determined with the acceptance %RSD values of less than 2%. The highest %RSD value was 1.09% which suggested the method to be acceptably precise. The limit of detection (LOD) and limit of quantification (LOQ) values were recorded to be at 0.016 and 0.196 mg/mL respectively. The % recovery of 4 known concentrations indicated the accuracy of the method was high with the recovery range between 98.66% and 101.8%. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines. This quantification method was a promising approach to provide a rapid and cost effective in evaluating gentamicin concentration for in-vitro applications.

Method Development and Validation using UV Spectrophotometry for Nigella sativa Oil Microparticles Quantification.

Nigella sativa oil (NSO) has been exploited for medical purposes for many generations. The fabrication of microparticles containing NSO intended for sustained release was done to be used in treating osteomyelitis. Method in quantifying NSO using UV-spectroscopy was developed and validated. Linearity shown a good correlation coefficient with the values higher than 0.995, both for actual and different analysts. The LOD and LOQ values were recorded to be 2.89 μg/mL and 8.75 μg/mL respectively. In addition, the highest %RSD values for the intermediate and repeatability studies were 0.970% and 0.445% which suggested the method was precise. The percentage recovery for 4 known concentrations gave the range between 98.16% to 99.39%, indicating the high accuracy of the method. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines.

Evolution of disease and potential biocontrol activity of Trichoderma SP. against Rhizoctonia solani on potato / Potencial do biocontrole por Trichoderma em relação à evolução da doença causada por Rhizoctonia solani em batata

Black scurf and stem canker disease cause by the fungal pathogen of Rhizoctonia solani and it is an economical important disease of potatoes in Bangladesh and throughout the world. This study evaluated the black scurf and stem canker disease development in potato and antagonistic activity of Trichoderma spp. against R. solani. The artificial infections were carried out using the inoculums of R. solani. The treatments (%inoculum) were: T1 (0% inoculum), T2 (5% inoculum), T3 (10% inoculum), T4 (20% inoculum), T5 (50% inoculum), and T6 (100% inoculum). The infection of stem canker and black scurf on progeny tubers increased with increase in inoculum levels. The highest disease incidence and severity was found in T6 (100% inocula). T6 showed the maximum black scurf infected tubers (russet, deformed and sclerotia). The lowest germination percentage, plant height and tuber yield were also obtained in the same treatment (100% inocula). Trichoderma spp reduced the growth of R. solani and the highest growth suppression was noted in isolate TM12. According to antagonistic activity, Trichoderma spp. reduced the growth of R. solani but was not able to stop the pathogen development. This finding showed management of this disease or R. solani invasion requires an integrated approach compared to Trichoderma single approach.

A rizoctoniose ou crosta negra causada por Rhizoctonia solani é a mais importante doença nos campos de batata em Bangladesh, bem como em várias regiões do mundo. Este trabalho avaliou o potencial do biocontrole com Trichoderma spp. e sua ação antagonista contra R. solani em batateira. Realizou-se as avaliações do potencial antagonosta usando inoculação artificial de R. solani. Os tratamentos (% de inóculo) foram: T1 (0% de inóculo), T2 (5% ), T3 (10%), T4 (20%), T5 (50%) , e T6 (100% de inóculo). A infecção de rizoctoniose na haste e crosta negra nos tubérculos aumentou proporcionalmente com o aumento do nível de inóculo. A maior incidência e severidade da doença ocorreu no tratamento 6 (100 % de inóculo), o qual apresentou maior quantidade de tubérculos infectados e deformados com escleródios em sua superfície. A menor porcentagem de germinação e produção de tubérculos também foi encontrada no tratamento 6, o qual também apresentou menor altura de planta. Trichoderma spp reduziu o crescimento de R. solani e a maior atividade de supressão do crescimento foi encontrada pelo isolado TM12. Foi detectada a atividade antagonista de Trichoderma spp. em reduzir o crescimento de R. solani, mas este não inibiu o crescimento total do patógeno. Conclui-se que o manejo da rizoctoniose da batateira por colonização de R. solani necessita táticas de manejo integrado em detrimento do uso isolado do manejo ou biocontrole com Trichoderma spp.