ABSTRACT
This study aimed to isolation of Acinetobacter spp, detect resistance patterns of isolated strains, phenotypic and genotypic detection of Metallo-beta-lactamase and typing of isolated strains from clinical and environmental respectively. this study includes 440 different clinical specimens, 672 environmental samples, inoculated on different culture media, confirmation of isolates by API20NE, PCR to detect 16SrRNA-23SrRNA gene, determine susceptibility pattern of isolates to different antibiotics and phenotypic and Genotypic detection of bla -OXA 51-like gene. 24 strains of Acinetobacter [5.45%] were isolated from 440 clinical samples, 27 strains of Acinetobacter were isolated from 672 environmental samples [4.017%]. Tetracycline was the most active drug against multi-drug resistant A.baumannii. [48/51 or 94%] of Acinetobacter isolates showed increase in zone of inhibition around IPM/EDTA disc compared with IPM disc alone. [49/51 or 96%] of Acinetobacter isolates were detected by presence of 16srRNA - 23 srRNA gene [universal gene present in all Acinetobacter species]. [49/51 or 96%] of isolated Acinetobacter spp showed band with blaOXA-51-like" genes. [37/51 or 72.5%] Acinetobacter isolates showed positive bands for class I integrase gene [gene responsible for multi drug resistance and outbreaks in hospitals]