Chemical rumenitis and septicemic colibacillosis causes death in a shoka deer calf [capreolus capreolus capreolus]

In May 2007, a suborder Shoka deer calf about one month of age was found in a preserved area of Arasbaran in the East Azerbaijan province. From an ecological view point, the Arasbaran area is uniquely labelled as a reserve biosphere. He calf had diarrhea that recovered after treatment. During rearing, The calf received pasteurized- homogenized milk from market as well as milk of a goat. After 3 weeks, the animal suddenly showed acute depression, anorexia and hypothermia. Antimicrobial and supportive therapy was ineffective and the calf died 12 hours after showing clinical signs. At necropsy, chemical rumenitis and hemorrhagic enteritis was observed. Pure Escherichia coli was isolated from liver, lungs, rumen and small intestine contents. Histopathologic examination revealed severe chemical rumenitis and necrohemorrhagic enteritis. Absence of mother, intake of milk from other species and stress of capture were predisposing factors. Entry and fermentation of milk in rumen provoked chemical rumenitis and stasis of digestive system could be responsible for septicemic Colibacillosis [colisepticemia] and death in this Shoka calf

[Pathological study of arthritis induced by type 2 bovine collagen and freund's complete adjuvant in rat]

Because of the pathological similarities between collagen induced arthritis [CIA] and rheumatoid arthritis, the CIA model has been the subject of extensive investigations. This study initiated to evaluate the pathologic lesions of induced arthritis by collagen in a total of 50 female rats [Rattus norvegicus.]. Arthritis was induced by two intrademial injections [14 days interval] of type 2 bovine collagen solution and Freund's complete adjuvant [FCA] into the tail and right leg paw of 40 rats. Severity of arthritis in affected leg was represented in grade to 4. Three weeks after beginning of the experiment, 22, 12, 5 and 1 affected rats were clinically diagnosed with 1, 2, 3 and 4 grades of arthritis respectively. Rats were intradermally injected into the tail showed only slight and moderate [grade 1 and 2] arthritis. Macroscopically, the involved joints were enlarged, swollen and stiff and deformity and ankylosis of affected joints were noticed in severe cases. Deep healing ulcers in planter of the paw were diagnosed in 14 rats. Histopathologic examinations revealed infiltration of mononuclear inflammatory cells and papillary hyperplasia of synovial membrane in mid and moderately affected animals [grade 1 and 2] and erosion of articular cartilage with formation of fibrovascular pannus on its surface in synovial membrane in severe cases [grade 3]. In very severe cases [grade 4], complete destruction of articular cartilage and access of granulation tissue to subchondral bone were noticed. Pathologic lesions of the involved joints were very similar to those reported from rheumatoid arthritis in human, erosive polyarthritis in dog and caprine arthritis/encephalitis

report on bovine fetal ectopia cordis cervicalis associated with two cervical sacs

A Holstein heifer with symptoms of dystocia was admitted in the Large Animal Clinic at the University of Tabriz. In the vaginal examination, the fetus was found at posterior presentation and by doing some obstertrical maneuvers, a male calf was delivered. An interesting phenomenon was the presence of two skin sacs in dimensions of 12 x 12 cm and 12 x 18 cm at the right and left upper cervical regions, respectively; and also the heart beats under the neck skin at the lower cervical region. Due to severe dyspnea, after 2 min the calf died. At necropsy, the heart was located outside of the thoracic cavity, under the neck skin. The direction of the heart axis was reversed as the base was directed caudoventrally and the apex craniodorsally. Unlike a normal calf's heart, the shape of its apex was rounded like a dog heart. Histopathologic examination revealed severe congestion, edema and hemosiderosis in the lungs, passive venous congestion and scattered foci of cardiomyolysis in the myocardium. Based on anatomical and histopathological findings, the disorder was diagnosed as bovine fetal Ectopia cordis cervicalis associated with two cervical sacs

Vaginal fibrosarcoma in cow [A case report]

A five-year-old Holstein cow with a history of vaginal prolapse was admitted to the Large Animal Clinic, Faculty of Veterinary Medicine, University of Tabriz. According to the owner, she had a 5-month history of vaginal prolapse and frequent sanguineous discharge from the vagina. Appetite was normal and its last parturition was two months ago. Clinical examination revealed a tumor like mass that attached to the vaginal roof and partly protruded through the vagina without any vaginal prolapse. After epidural anesthesia, the mass was surgically removed with some of the surrounding tissue. The dimensions of the mass were 5.2 x 4 x 3.4 cm. Macroscopically, it was relatively well circumscribed with a lobulated and ulcerated surface. In cut section, the mass was homogenously creamy in color. There were no foci of necrosis or hemorrhage. Microscopically, the growth was composed of spindle-shaped flbroblastic type tumor cells that formed interlacing and intersecting bundles. The neoplastic cells showed pleomorphism, karyomegaly and slight nuclear hyperchromatism. The mitotic index was less than five. Based on the site, biological behavior, immunohistochemistry results, macroscopic and microscopic characteristics, the mass was diagnosed as a well-differentiated fibrosarcoma. This is the first report of cow vaginal fibrosarcoma from Iran

Molecular and pathological study of bovine aborted fetuses and placenta from Neospora caninum infected dairy cattle

The objective of the study was to evaluate the presence of Neospora caninum organisms in the brain of aborted fetuses and placentas of full-term calves born of seropositive cows. During 2006-2007, 12 brains of aborted calves from Neospora seropositive cattle and 7 placentas from seropositive dams giving birth to full-term calves, from four dairy cattle farms located around Tehran province, Iran were examined by Nested-PCR and histopathology techniques. The Nested-PCR demonstrated that all of 12 aborted fetal brain samples and 5 of 7 placentas were infected by N. caninum. Mild to severe placentitis was observed in 5 placentas. Severe hyperemia and perivascular and perineuronal edema revealed in all fetal brain. In 3 out of 12 brains, scattered foci of hemorrhages, neuropilar necrosis and gliosis were present. In addition, nonpurulent encephalitis with severe lymphohistiocytic perivascular cuffing in one case and a small tissue cyst like Neospora caninum cyst in other calf were observed. Our results confirmed the molecular and histopathologic findings of other studies about Neospora caninum infection and it seems to support the hypothesis that Neospora infection is associated with bovine abortion in Iran

Trichoblastoma [basal cell tumor] in a rabbit

Diagnosis of a subcutaneous mass in a rabbit. Case report. A 3-years-old female mixed shorthair black and white rabbit. The rabbit was referred to the Small Animal Clinic, Faculty of Veterinary Medicine, University of Tehran, with a 1-month history of a cutaneous mass. Clinical examinations, radiography of thorax and ultrasonography of abdominal cavity was carried out. The mass was removed surgically. Clinical examinations revealed a well circumscribed subcutaneous mass on left gluteal region. Macroscopically the mass was solid, ovoid [1.6x1.3 x 0.6 cm] with reddish cut surface and flesh-like consistency. Histopathologic examination revealed prominent, oval, basophilic nuclei, with single scant nucleolus and eosinophilic cytoplasm. The tumor cells morphologically resembled the normal basal cells of the epidermis and showed no epidermal or adenexal differentiation. This is the firstreport of rabbit trichoblastoma from Iran, [basal cell tumor]

pathological study of hepatic lesions after experimental aflatoxicosis in broiler chickens

To study gross and histopathologic lesions and also relative weight of liver in experimental aflatoxicosis in Ross broiler chicks. Experimental study. Two hundred and forty 1-day-old Ross broiler chicken. Linear model analysis and Duncan's method for mean values with S AS package. The chickens were fed by NRC [1994] diet. Feed and water were provided ad libitum. The diets were divided into 3 groups: control [0 or basal] and treatment with 1 and 2 ppm of aflatoxin. Aflatoxin was produced by infecting of autoclaved rice with Aspergillus parasiticus NRRL-2999 in the flasks and titrated by TLC and HPLC. After 21 and 42 days, three chickens from each group randomly killed and their livers were weighed. Tissue samples were collected and fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned at 5 um and stained by haematoxylin and eosin [H and E]. Relative weights of the livers [g/l00g.b.w] in treatment groups were significantly increased as compared with control [p<0.05]. Histopathologic examination revealed severe fatty change, regeneration foci of liver cells, fibrosis of portal regions and bile ductule hyperplasia. The lesions were very severe in 42-days-old chickens and had the lesser severity in 21-days-old chickens. Liver is the target organ for aflatoxin. Aflatoxin causes severe lesions in the liver and increases its relative weight.Prolonged exposure to low concentrations of toxin produces severe changes in fat metabolism and bile ductules proliferation