ABSTRACT
Different strains of Echinococcus Granulesus have been found in endemic areas of Iran. This variation has a significant aspect in the field of medical parasitology and veterinary and also in epidemiology, pathology, control and prevention of Hydatid cyst infection. Morphology, epidemiology and biochemical studies can be carried out to determine Hydatid cyst strains in human and animal origins as host. This study was done to measure different biochemical compositions of liver Hydatid cyst fluids in human, sheep, goat, cattle and camel in 2004.In a cross sectional-analytical study, 112 samples of Hydatid fluids were collected from the liver cysts of different hosts: 16 sheeps, 64 catties, 12 goats and 10 camels in slaughter houses of Sari and Ghaemshahr and 10 human in Imam hospital. All cyst fluids were centrifuged at 4500 rpm at 4°C for 45 minutes and the supernatants were analyzed for various biochemical parameters.Quantitative differences were observed in the levels of Sodium, Glucose, Urea, Alanin Aminotransferase [AST] in liver cystic fluids obtained from different hosts, although these differences were not statistically insignificant. However, differences in the levels of Potassium, Calcium, Triglycerides, Cholesterol, Uric acid, Creatinin, Albumin, Gamma Glutamyl Transferase, Aspartat Aminotransferase [AST] and Creatinine Phosphokinase [CPK] in different Hydatid cyst fluids were statistically significant [P<0.05].Differences in biochemical composition of different Hydatid cyst fluids suggest the possible existence of more than one strain of Echinococcus Granulosus in human and other intermediate domestic animal hosts in Mazandaran
ABSTRACT
Background and Objective: Regarding prospective effects and cross immunity between some species of parasites, obtained antigens of a parasite can be used to prevent other parasitic diseases and adjuvant of this effect can be increased. The aim of this study was to evaluate the effect of hydatid cyst components in prevention of H. Nana in rat
Methods: 40 rats with the same sex and age were selected after gaining confidence in nonexistence of any parasitic disease. They were divided into 2 groups and each group were divided into 4 subgroups. In one group, antigen of fluid, protoscolex, germinal layer were injected respectively and in the other group, these antigenic materials were used with an adjuvant. Control group was considered for both groups. The immunization was performed by multiple injection. One week after injection of antigenic materials, H. Nana eggs were received orally by rats. After observation of H. Nana in stool of rats in control group, blood sample was taken from all rats. Different biochemical tests and immunological and parasitological tests were performed on sera and stool samples, respectively
Findings: Different protein measurements especially gammaglobulin indicated that membrane of hydatid cyst had the strongest antigenic effect in comparison to 2 other parts of cyst and hydatid cyst fluid showed the weakest one
Conclusion: Stool test in control group and exposing egg showed that this group was infected. It was not seen any exposing egg in rats that immunized with different hydatid cyst components. Statistical and biochemical results demonstrated that this effect increased by using adjuvant with different hydatid cyst components and its results showed that immune effect would be increased after injection of adjuvant