ABSTRACT
Rosa damascene Mill. is one of the most important medicinal and aromatic plants mountainous areas Kashan. This plant have different genotypes that they are very various in size and colour of flowers, number of petals, amount of essential oil and components. Extraction and determine of essential oil combination of three genotypes from Rose of Kashan area. The flowers of these three genophytes fully double link, white and yellow was collected from mountainous areas Kashan. The essential oil, by hydrodistillation using an all-glass Clevenger-type apparatus. The analysis of the oils were performed by using GC and GC-MS methods. Yields of essential oil of fully double link genotype was 0.025% [v/w] and 39 components was identification. The major components of this genotype were citronelool[%34.7], nonadecane[%14.5] and heneicosane [%10.3]. Yields of the essential oil of white genotype was 0.035% [v/w] and 21 components was identification, that major components were found as citronellol [%53.61], nonadecane [%17.75] and geraniol [%12.59]. Yields of the essential oil of yellow genotype was 0.7% [v/w] and 29 components was identification, that major components were found a s heneicosane [%32], nonadecane [%30] and 9 -nonadecene [%10.5]. Monoterpene citronelool was more than 30-50% from essential oil of genotypes fully double link and white, but this component wasn't in yellow genotype. On the other hand, heavy hydrocarbon component was more than 50% of essential oils component of yellow genotype, but amount of this component was 17% in fully double link and white genotypes
ABSTRACT
Artemisia persica is a common species in South East Iran and A. persica is the only species in this genus and the species name persica means from Iran. Artemisia persica have been used in traditional medicine to treat various infections and inflammations. It is an effective remedy for S. aureus and B. subtilis bacterial infection. The selected methanol extracts were screened against two strain of bacteria Staphylococcus aureus and Bacillus subtilis at seven different extracts concentrations [5, 25, 100, 150, 200, 300 and 400 micro g/ml] using disc diffusion and wheel assay methods. The minimum inhibitory and bactericidal concentrations [MIC, MBC and Death kinetic] of the active extracts were tested using MH broth-dilution methods. For standard strains of S. aureus the extracts for MIC were range 100 mg/ml and MBC range were 150-200 micro g/ml. For B. subtilis standard strain the MIC extracts range were150-200 micro g/ml and MBC were 400 micro g/ml, Kinetic of death were 4 hours for B. subtilis compared to 5 hours for S. aureus. These results suggest that extracts of Artemisia persica may have the antibacterial activity against some pathogenic bacteria. It has an antibacterial action and inhibiting the growth of Staphylococcus aureus and Bacillus subtilis and it may be attributed to its content of the artemisinin, tannins, saponin, alfa- pinene and camphor. From this study it can be concluded that many extracts possess antibacterial activity Plant extracts have been used for many pharmaceuticals, alternative medicine and natural therapies