[Preliminary study on frequency of mycoplasmal mastitis in some dairy cattle herds in Mashhad Suburb, Iran]

To determine sublicnial and clinical mycoplasmal mastitis and their frequency in dairy cattle herds. Prospective study. One hundred Holstein dairy cattle. Affected cows with clinical [n=19] and subclinical mastitis [n=81] were determined by using routine clinical methods and California Mastitis Test [CMT]. Samples were collected in the sterile tube containing Ampicillin. They were inoculated into both PPLO broth and PPLO agar for 96 hours and one week at 37 [degree sign] C, respectively. Chi-square test. Mycoplasma was isolated from 13 cows. The frequency rate of clinical and subclinical mastitis were 15.38% and 84.61%, respectively. There were no significant correlation between infected quarter and herd population [P>0.05]. The mammary glands of dairy cattle herds in suburb of Mashhad-Iran were infected by Mycoplasma. It is necessary to be investigated more in the field of mycoplasmal mastitis in dairy cattle herds

survey of Histophilus somni and other bacteria frequency in mastitis of Holstein dairy cattle herds in suburb of Mashhad

1-If Histophilus somni can be involved ofmastitis in Holstein dairy cattle. 2- The frequency ofHistophilus somni and other bacteria isolated in mastitiscases. Cross-sectional. 116 Holstein dairy cattle. Cows were examined in 6 dairy cattle herds insuburb of Mashhad, Iran. They were tested for clinical [No:22; 18.64%] and sub clinical mastitis [No: 94; 81.04%] byusing routine clinical methods and California Mastitis Test [CMT]. They were cultured in Brain Heart Infusion Yeastagar at 37° C in 10% Co2 for Histophilus somni and otherbacteria infection. They were evaluated after 24- 48 hours. Chi- square and Fisher tests.Histophilus somni was isolated from only 2[1.73%] clinical mastitis cows. Sub clinical mastitis cows were notinfected by Histophilus somni. There were significantdifferences between clinical and sub clinical mastitis inrespected to mammary gland infection [P<0.05]. Theinfection rate in winter and spring seasons were 1.73% [No:58] and [1.73%], respectively. However, 8 bacteria wereisolated, too. The infection rate of mastitis due toHistophilus somni was very low [1.73%]. It was cultureddifficulty. The real infection rate may be higher. It isnecessary to use more sensitive methods