ABSTRACT
Aim: The aim of this study was to investigate the VRE frequency and the rate of each gene in isolated enterococci frompatients with intestinal infection in the central region of Iran
Background: Enterococci infections are a public health growing concern due to the glycopeptide antibiotics resistanceespecially vancomycin. Genes, vanA, B, and H contribute to the influence of vancomycin-resistant enterococci [VRE]
Patients and methods: This study was conducted from January to July 2014 in Shahrood university hospital.Enterococci isolation and its antibacterial susceptibility were performed by culturing in Aesculin Azide agar and Kirby-Bauer method, respectively. Vancomycin-resistant genes were screened through conventional PCR, and subsequentlysequenced
Results: Among 265 specimens, 100 isolates revealed enterococci, in which E. faecalis [91%] and E. faecium [9%]. Theisolated enterococci were resistant to vancomycin [6%] and chloramphenicol [21%], whereas their large proportions [94% to100%] were multi-drug resistant. All VRE isolates belonged to E. faecalis, conversely, the E. faecium were susceptible to thesame antibiotic. Both vanA and vanH genes were identified in all VRE isolates, although, no vanB gene was indicated.Homology analysis of sequenced amplicons verified the full length compatibility to the worldwide reported genes
Conclusion: The present study revealed VR E. faecalis in gastroenteritis patients and resistance factor for vanA andvanH genes are coordinated. Since enterococci isolates were all multidrug resistance, increase in VR E. faecalis vanA /vanH in this area could be expected