ABSTRACT
BACKGROUND: Glycosylation process helps in stabilization and solubilization natural of compounds. Glycosyltransferase (YjiC) provides for high efficient glycosylation product with an incredible variety of sugar moieties, typically from UDP-glucose. Vitamin C (L-ascorbic acid) is an essential nutrient for humans and certain other animal species. Vitamin C functions in many biological processes, such as collagen synthesis, antoxidation, intestinal absorption of iron. UDP-glucose acts as a starting material for glycosyltransferase (YjiC). In order to recycle UDP-glucose after glycosylation with glycosyltransferase (YjiC), sucrose synthase (AtSUS1) carry out than reversible conversion of sucrose and UDP to UDP-glucose and fructose. MATERIALS AND METHODS: DNA was extracted than E.coli BL 21 and E.coli JM 109 hosts were used for expression of proteins. The purified protein was then analyzed by 12% SDS-PAGE than used for enzymatic recycle system. TLC analyse of the products were carried out to the test glycosylation. RESULTS: In this study, we choose substrate vitamin C for the enhancement of enzymatic recycling system glycosylation. In this recycle system due to the high concentration of sucrose and vitamin C but low concentration of UDP-glucose with is relatively expensive made the system more economic. TLC analyses of the products were carried out to the recycled system worked and glycosylation product. CONCLUSION: Based on proved function an enzymatic recycling system with glycosyltransferse (YjiC) and sucrose synthesis (AtSUS1) to be applicable to enzymatic production of vitamin C glucoside and resveratrol glucoside. Further analysis by HPLC and MS will elucidate the products.