The effects of Bio-Oss(R) as a scaffolds during sinus bone graft using mesenchymal stem cells in rabbit

Mesenchymal stem cells (MSCs) have been though to be multipotent cells that can replicate that have the potential to differentiate into lineages of mesenchymal tissue including the bone, cartilage, fat, tendon, muscle, and marrow stroma. Especially, scaffolds to support cell-based tissue engineering are critical determinants of clinical efforts to regenerate and repair the body. Selection of a matrix carrier imvolves consideration of the matrix's role as a scaffold for physical support and host tissue integration as well as its ability to support of synergize the osteoinductive program of the implanted mesenchymal stem cell. The aim of this study is to evaluate the effect of autobone and Bio-Oss(R) to adherent mesenchymal stem cells as scaffolds on sinus augmentation with fibrin glue mixture in a rabbit model. 16 New Zealand White rabbits were divided randomly into 4 groups based on their time of sacrifice (1, 2, 4 and 8 weeks). First, mesenchymal stem cells were isolated from iliac crest marrow of rabbits and expanded in vitro. Cell culture was performed in accordance with the technique described by Tsutsumi et al. In the present study, the animals were sacrificed at 1, 2, 4 and 8 weeks after transplantation, and the bone formation ability of each sides was evaluated clinically, radiologically, histologically and histomorphologically. According to the histological observations, autobone scaffolds group showed integrated graft bone with host bone from sinus wall. At 2 and 4 weeks, it showed active newly formed bone and neovascularization. At 8 weeks, lamellae bone was observed in sinus graft material area. Radiologically, autobone with stem cell showed more radiopaque than Bio-Oss(R) scaffolds group. there were significant differences in bone volume between 4 and 8 weeks (p<0.05).

Treatment of Long Tubular Bone Defect of Rabbit Using Autologous Cultured Osteoblasts Mixed with Fibrin / 대한정형외과연구학회지

PURPOSE: The osteogenic potential of autologous cultured osteoblasts mixed with fibrin when transplanted to bone defects was evaluated. MATERALS AND METHODS: Radial shaft defects over 15 mm were made in 30 New Zealand white rabbits. Fifteen rabbits in the control group underwent an iliac bone graft and 15 rabbits in the experimental group underwent an autologous cultured osteoblast injection mixed with fibrin. Both groups were compared radiologically and 5 rabbits in each group were sacrificed for histological evaluation using H-E and Masson's trichrome stains at 3, 6, and 9 weeks. RESULTS: Osteogenesis in the control group progressed more rapidly than in the experimental group. However, at 9 weeks, bone formation in both groups were similar and showed no significant difference in terms of the amount of bone formation and the quality of bone union. CONCLUSION: Autologous cultured osteoblast transplantation mixed with fibrin in bone defects was found to produce bone efficiently.

Bone Formation of Cultured Osteoblasts in Bone Defect of Radial Shaft of Rabbit / 대한정형외과학회잡지

PURPOSE: To evaluate the osteogenic potential of an autologous cultured osteoblast transplant to the bone defects. MATERIALS AND METHODS: Radial bone defects over 15 mm were made in 20 New Zealand white rabbits using the anterior approach. There were 10 rabbits in the control group, which underwent an iliac bone graft to the preformed bone defect 3 weeks from the initial operation. There were 10 rabbits in the experimental group that underwent an autologous cultured osteoblasts injection. After 9 weeks, both groups were compared radiologically and histologically. RESULTS: The osteogenesis in both groups were progressed similarly and there was no difference in terms of the amount of bone formation and the duration of the bone union. CONCLUSION: An autologous cultured osteoblast transplant to the bone defect produces bone efficiently.In addition, it can be applied to a wide field, which requires a bone grafting operation.

Clinical Applications of Autologous Cultured Osteoblasts: Case Report

We treated 3 cases of fracture and 1 case of avascular necrosis of femoral head using autologous cultured osteoblasts injection. The stromal cells from the bone marrow were cultured to differentiate to osteoblasts for 4 weeks. The fracture sites of each patients were right ulnar shaft, left radial shaft, and left 5th metatarsal base. All of the fractures showed callus formations after 1 week of osteoblasts injection to the fracture site. After 4 weeks, callus formations were progressed. Avascular necrosis of femoral head was bilateral and both were Ficat stage II. Core decompression and allograft impaction were performed to the left, and core decompression and autologous cultured osteoblasts injection percutaneously after 4 weeks of the decompression operation were done to the right femoral head. CT images of 1 year from the operations showed trabecular bone formation and well maintained femoral head contour of the right femur, but resorption of the grafted bone for the left.