ABSTRACT
Extraskeletal osteosarcoma is a rare malignant tumor of soft tissue. In addition, there are only several cases of osteosarcoma of the mediastinum as a primary site in the world. We report a case of extraskeletal osteosarcoma arising in the posterior mediastinum. A 54-year-old man visited our hospital because of chest pain. Chest CT showed a 12 cm sized huge mediastinal mass. The mass revealed solid and ivory-colored cut surface with multifocally ovoid cystic spaces and hemorrhage. Microscopically, the tumor consisted of polygonal stromal cells, giant cells and varying amounts of neoplastic new bone, and mitotic figures were frequently observed. There were no distant metastasis and regional lymph node involvement.
Subject(s)
Humans , Middle Aged , Chest Pain , Giant Cells , Hemorrhage , Lymph Nodes , Mediastinum , Neoplasm Metastasis , Osteosarcoma , Stromal Cells , Tomography, X-Ray ComputedABSTRACT
Ultraviolet (UV) radiation is the main physiological stimulus for human skin pigmentation. Recently, nitric oxide (NO) have been involved in mediation of skin pigmentation induced by UVB. Rutin, a flavonoid of vegetables and fruits, has antiviral and antioxidant properties. Therefore, we investigated the effect of rutin on UVB-induced melano-genesis and NO production in HM3KO cells. In this study, we demonstrated that UVB-irradiation stimulated melanin content and tyrosinase activity in HM3KO cells. Rutin suppressed UVB-stimulated total melanin content and tyrosinase activity in a dose-dependent manner. Additionally, we showed that UVB-irradiation stimulated NO production in HM3KO cells. Rutin also suppressed UVB-induced NO production and repaired reduction of cell proliferation by UVB. UVB stimulation of melanogenesis was mimicked by exogenous NO donor (sodium nitroprusside, SNP), and rutin effectively suppressed it. Therefore, we concluded that rutin suppressed UVB-stimulated melanogenesis and that it is involved in melanogenesis regulation partially through the suppression of UVB-induced NO production.
Subject(s)
Humans , Cell Proliferation , Fruit , Melanins , Melanoma , Monophenol Monooxygenase , Negotiating , Nitric Oxide , Nitroprusside , Rutin , Skin Pigmentation , Tissue Donors , VegetablesABSTRACT
PURPOSE: Since 1971, Nitrofen (2,4-dichloro-4`-nitrodiphenyl ether) herbicide has been known to induce variable congenital abnormalities in rats. However, until now there has been no animal model of congenital diaphragmatic hernia (CDH) using this herbicide in Korea. Therefore, CDH in rats using Nitrofen was tried for searching on pathogenesis or using therapeutic modalities of CDH. METHODS: 20 Sprague-Dawley rats ingested Nitrofen as indicated on the 9.5th day of gestation, and after sacrificing on the 21th day of gestation. 243 offspring were harvested for the examination of diaphragmatic herniations. Dissections were made along sites of herniations to confirm the diaphragmatic herniation's site and size, lung/body weight ratio comparison as well as to accomplish microscopic radial saccular counting and evaluation of alveolar septal wall maturations and a comparision was made between the groups. RESULTS: CDH appeared in 149 of 243 offspring. CDH only appeared in the posterior portion of the diaphragm regardless of size or the side of appearance. Left sided CDH was the most common single diaphragmatic anomaly, represented in 112 offspring. Right sided CDH was next, found in 31 offspring, and bilateral CDH was seen in 6 offspring. Left sided CDH was almost always associated with visceral herniations and with pulmonary hypoplasia on the affected side, which was proved in the decreased Lung/Body weight ratio and in decreased radial saccular counts and in increased alveolar septal wall thickness in the affected lungs of CDH. In94 offspring without CDH following exposure to Nitrofen, moderate pulmonary hypo plasia was microscopically observed. CONCLUSION: Initial Rat modeling of congenital diaphragmatic hernia using Nitrofen showed results of herniation and pulmonary hypoplasia of the affected lungs acceptable for further experimental studies on CDH and accompaning pulmonary abnormalities.
Subject(s)
Animals , Animals , Pregnancy , Rats , Congenital Abnormalities , Diaphragm , Hernia, Diaphragmatic , Korea , Lung , Models, Animal , Rats, Sprague-DawleyABSTRACT
To select high risk group in middle cerebral artery(MCA) infarction by comparing and analysing 10 risk factors in above patients, we analyzed 32 MCA infarcted patients and 30 case control groups retrospectively. Through the analysis of 10 risk factors(age, heart disease, blood pressure, total cholesterol, triglyceride, HDL-cholesterol, glucose, smoking, r-GTP, past history : hypertension or cerebrovascular accident) in MCA infarction by the times patients arrieves in emergency room by scoring system made by author, MCA infarcted patients showed score about 1.4 in 24 cases(75.00%) among 32 MCA infarction patients, and in control group, score below 1.4 showed in 28 cases(93.33%) among 30 cases. By the scoring system made by author, the higher risk grouped patients could identified more easily in emergency department.
Subject(s)
Humans , Blood Pressure , Case-Control Studies , Cholesterol , Emergency Service, Hospital , Glucose , Heart Diseases , Hypertension , Infarction , Infarction, Middle Cerebral Artery , Retrospective Studies , Risk Factors , Smoke , Smoking , TriglyceridesABSTRACT
No abstract available.