ABSTRACT
Statement of the Problem: Recurrent aphthous stomatitis [RAS] is one of the most common lesions in the oral cavity. Due to its multifactorial nature, there is no definitive treatment for RAS. Laser therapy is one of the suggested treatments to reduce patient's discomfort
Purpose: The purpose of the present clinical trial is to assess the effect of low and high level laser therapy on pain control and wound healing of RAS
Materials and Method: Thirty six patients with minor RAS were divided into three groups. Group 1 [n=14] received CO[2] laser, group 2 [n=12] were treated with In-GaAlP Diode laser and group 3 [n=10] received sham laser as placebo. All patients were evaluated daily up to 15 days after receiving one session of laser therapy. Pain severity before and after treatment, wound healing, patient's satisfaction, and functional disturbance before and after treatment were recorded for each patient
Results: According to statistical analysis, pain reduction after treatment in group 1 was 7.00+/-2.41, in group 2 was 2.08+/-2.31, and in group 3 was 1.40+/-1.77. In addition, a significant difference was observed in the reduction of functional complications in CO[2] laser treated patients compared to the other two groups
Conclusion: High-level laser treatment showed analgesic effects on RAS, but no healing was observed. Low-level laser therapy demonstrated no positive effect on recurrent aphthous ulcers
ABSTRACT
Statement of the Problem: Oral candidiasis is the most common opportunistic infection affecting the human oral cavity. Photodynamic therapy, as one of its proposed treatment modalities, needs a distinct dye for achieving the best effect
Purpose: The purpose of this study was to evaluate photosensitization effects of four distinct dyes on standard suspension of Candida albicans [C. albicans] and Candida dubliniensis [C. dubliniensis] and biofilm of C. albicans considering the obtained optimum dye concentration and duration of laser irradiation
Materials and Method: In this in vitro study, colony forming units [CFU] of two sets of four groups of Laser plus Dye [L+D+], Dye [L-D+], Laser [L+D-] and No Laser, No Dye [L-D-] were assessed individually with different methylene blue concentrations and laser irradiation period. The photodynamic therapy effect on standard suspension of Candida species [using methylene blue, aniline blue, malachite green and crystal violet] were studied based on the obtained results. Similar investigation was performed on biofilm of C. albicans using the spectral absorbance. Data were imported to SPSS and assessed by statistical tests of analysis of variance [ANOVA] and Tukey test [alpha= 0.05]
Results: CFU among the different dye concentration and irradiation time decrease in dose- and time-dependent manner [p> 0.05], all of which were significantly lower than the control groups [p< 0.05]. Among the examined photosensitizers, there was no statistically significant difference, [p> 0.05] though all of them were significantly decrease CFU compared with the control groups [p<0.05]. In L+D- and L+D+ groups, biofilm was significantly destroyed more than that of L-D- [p< 0.05]
Conclusion: Photodynamic therapy might be used as an effective procedure to treat Candida associated mucocutaneous diseases and killing biofilm in the infected surfaces such as dentures