ABSTRACT
Background: Peripheral arterial disease is known to be associated with the diabetes and ankle-brachial pressure index (ABPI) can be used to screen such patients having PAD. Aims & Objective: To highlight the application of ankle brachial pressure index as a routine procedure and to assess the feasibility of using ABPI in detecting PAD in the patients of diabetes mellitus. Material and Methods: 30 Diabetic and 30 Non diabetic patients were recruited. Details regarding anthropometric measurements, blood sugar levels, lipid profile, Edinburgh questionnaire, peripheral pulse examinations etc. were filled in preformed questionnaire. Ankle-brachial pressure index (ABPI) was measured with the help of Handheld Doppler machine. Descriptive statistics in the form of frequency and percentage is used for description of data. Chi-square test was used for comparison of events in two groups. Results: PAD was more common in old age but found not to be associated with gender. Body mass index, surface area and wait hip ration was significant more in PAD group as compared to non-PAD. All blood sugar parameters [Fasting blood sugar (FBS), postprandial blood sugar after 2 hours (PP2BS) and Glycosylated haemoglobin (HB1AC)] were significantly more in PAD group as compared to non-PAD. High density lipoprotein was significantly less in PAD group as compared to non-PAD. Triglyceride, Total Cholesterol and Low density lipoprotein was significantly more in PAD group as compared to non-PAD. ABPI was significantly less in diabetics as compared to non-diabetics. More diabetics were in having PAD as compared to non-diabetics. Conclusion: PAD is found to be associated with more weight, adverse lipid profile and diabetes. ABPI can be used to screen the PAD in diabetic patients.
ABSTRACT
Aim: To evaluate the performance of an automated BACTEC MGIT 960, a non-radioactive, non-invasive liquid culture system for cultivation of M. tuberculosis complex in terms of recovery rate and time. Materials and Methods: From March 2005 to December 2007, 14,597 specimens were processed using the MGIT 960 system and the results were compared with conventional L.J medium. We standardised r-nitro benzoic acid (PNBA) assay on MGIT 960 TB system for identification of M. tuberculosis complex and evaluated its usefulness by comparing the results with an in-house molecular assay and sequencing. Results and Discussion: Of the total 6143 (42%) isolates positive for M. tuberculosis complex, 6015 (41%) were positive by MGIT 960 TB system. In contrast, 3526 (24%) M. tuberculosis complex isolates grew on the conventional L.J medium. The mean turn around time for mycobacterial growth in smear-positive specimens was nine days for MGIT 960, and 38 days for L.J. medium whereas in smear negative specimens it was 16 days by MGIT vs. 48 days by L.J. Conclusion: MGIT 960 system with PNBA assay for identification of M. tuberculosis complex is a rapid and useful method in laboratories processing a large number of specimens.
ABSTRACT
2-methyl-3(3'-methyl-2'-pyridyl)-4 (3H) quinazolinone (SRC-820) and methaqualone inhibited the growth of Streptococcus faecalis-R (ATCC-8043). Phosphoenolpyruvate formation from 3-phosphoglycerate by an enzyme extract of S. faecalis was inhibited by SRC-820. With edetate or fluoride, an additive inhibitory effect by SRC-820 was observed.