Detection of rifampin resistant tuberculosis meningitis by polmerase chain reaction linked single strand conformation polymorphism analysis of cerebrospinal fluid

To rapidly detect rifampin resistance in Mycobacterium tuberculosis isolates causing meningitis in northeast Iran. This study presents the results of a polymerase chain reaction-single strand conformation polymorphism [PCR-SSCP] analysis for the evaluation of rifampin resistance directly from the CSF of 47 patients strongly suspicious to have tuberculosis meningitis in Emam Reza University Hospital, Mashhad, Iran over 3 years [2002 to 2005]. Each CSF sample underwent microscopic examination, culture and DNA amplification by 2 PCR protocols and subsequent detection of mutations by SSCP analysis. Among these patients, no mutations were revealed in the rpoB segment by SSCP. The SSCP analyses of these samples shows complete susceptibility to rifampin. The use of this method can radically reduce the time needed to provide clinicians with data useful in aiding the selection of appropriate drugs

Bacterial pattern of chronic rhinosinusitis fess candidates and their antibiotic susceptibility in Norhteast of Iran

Sinusitis is a major cause of illness in the world, and represents a substantial economic burden. Accurate diagnosis and appropriate antibiotics will optimize the! Chances of achieving and early cure. The main factors in choosing antibiotic therapy are Likely Infecting pathogens, and the pattern of bacterial antibiotic resistance, endoscopically directed middle meatal aspiration culture is increasingly used in this setting; but studies have not convincingly demonstrated the validity of this technique, So this study was undertaken over a 12-month period to identify causing pathogens of chronic sinusitis and their antibiotic susceptibility pattern in Northeast of Iran. Over a 12-month period, a total of 32 samples were taken from 16 patients diagnosed as having chronic bacterial rhinsinusitis. The samples were sent to the laboratory where a pathogen was identified and antibiotic susceptibilities were determined. Most bacteria isolated were aerobic or Facultative bacteria, gram negative rods as Entrobacter spp [18.5%], Klebsiella pneumonia [15.4%], E.Coli [9.23%] and Staphylococcus aureaus [15.4%] although appropriate for anaerobes cultivation were employed, anaerobes were identified in just 3 specimens the predorninant anaerobes isolate was Fusobacterium.. The results of this study cam give practicing physician's vital information about pathogen profiles and susceptilities within our communities and help them in making appropriate treatment choices for their patients with chronic rihinosinusitis

Evaluation of ceftazidime antimicrobial activity in infections caused by burns

In vitro evaluation of ceftazidime antimicrobial activity in infections caused by burn and its comparison to the other antibiotics is presented. In vitro tests for susceptibility to ceftazidime and other antibiotics were carried out on 744 bacterial strains collected from burn infections. The results have shown that generally ceftazidime was more active against Pseudomonas aeruginosa and other gram-negative bacilli like Klebsiella pneumoniae, Escherichia coli, and Proteus than amikacin, gentamicin, tobramycin, carbenicillin, kanamycin, and streptomycin in terms of potency and activity. In addition, and according to our in vitro studies we suggest that ceftazidime could be considered a valuable alternative to other antibiotics in the treatment of burn infections caused by P. aeruginosa and other gram- negative bacilli