ABSTRACT
The purpose of this study is to evaluate the effect of Curcuma longa extract on the telomerase gene expression in QU-DB lung cancer and T47D breast cancer cell lines. The present study is an experimental research. Using 3 different phases n-hexane, dichloromethane and methanol, total extract of Curcuma longa in a serial dilution was prepared and three phases was analyzed for determining which phase has more curcuminoids. Then the extract cytotoxicity effect was tested on breast cancer cell line [T47D], and lung cancer cell line [QU-DB] by 24, 48 and 72 h MTT [Dimethyl thiazolyl diphenyl tetrazolium] assay. Then, the cells were treated with serial concentrations of the extract. Finally, total protein was extracted from the control and test groups, its quantity was determined and telomeric repeat amplification protocol [TRAP] assay was performed for measurement of possible inhibition of the telomerase activity. Cell viability and MTT-based cytotoxicity assay show that the total extract of Curcuma longa has cytotoxic effect with different IC50s in breast and lung cancer cell lines. Analysis of TRAP assay also shows a significant reduction in telomerase activity on both cancer cells with different levels. Curcuma longa extract has anti-proliferation and telomerase inhibitory effects on QU-DB lung cancer and T47D breast cancer cells with differences in levels of telomerase inhibition
ABSTRACT
The present study investigated the role in transmission of M. tuberculosis strains isolated from tuberculosis patients residing in Northwest [East and West Azarbaijan] of Iran. We performed restriction fragment length polymorphism analysis on IS6110 of M. tuberculosis isolated from Northwest Iran. Total of 165 isolates of M. tuberculosis were analyzed by RFLP method. The 5 copies and more IS6110 isolates comprised 30.52% of the total isolates. They formed 16 clustered groups consisting of 2 to 10 cases each. 69.48% of patients had a unique RFLP patterns. Cases from male patients were more clustered than female patients but statistically was not significant [P>0.05]. In this study patients with 56 and older age were strongly associated with clustering [59.6%], which were significantly more than younger patients [P<0.05]. In the present study we found old age as a major risk factor in contact dependent transmission of TB compared to disease recurrence. Unemployment and poor living condition were also among the risk factors in transmission of tuberculosis