ABSTRACT
Objective: to detect H. Pylori antigen in stool and to determine the diagnostic accuracy of H. pylori stool antigen
Design: comparative analytical study
Setting: this study was carried out at Pakistan Medical Research Council [PMRC], at Jinnah Postgraduate Medical Centre, Karachi from May 2006 to September 2006
Material methods: endoscopic biopsy specimens were collected for rapid urease test and histopathology examination. Stool samples were taken for detection of antigen of Helicobacter pylori. The stool antigen test is a rapid immuno-assay based on "lateral flow chromatography technique" using monoclonal antibodies for qualitative detection of Helicobacter pylori antigen in human stool
Result: fifty males and 50 females were selected for this study by non-probable purposive sampling technique. Their mean age was 39 years ranging from 15 to 55 years? The sensitivity and the specificity of Helicobacter pylori stool antigen test [HPSA] with rapid urease test was found to be 89.1% and 92.6% respectively and the sensitivity and specificity of HPSA test with histopathology was 92.8% and 89.6% respectively
Conclusion: helicobacter pylori stool antigen [HPSA] is an accurate easily carried out in routine laboratory and is non-invasive