Translocation of the scleral suture knot on scleral-fixated posterior chamber intraocular lens

PURPOSE: To evaluate exposure rate of suture material at the transscleral suture fixation of posterior chamber intraocular lens (TSFPCIOL) with the method in which the scleral suture knots are trans positioned from both nasal and temporal sclera to superonasal. METHODS: With retrospective chart review 16 eyes of 15 patients who underwent TSFPCIOL with the transpositioned suture site, the authors checked their visual recovery rate and complicated disorders after the surgeries. RESULTS: Average corrected visual acuities at final visits improved to 0.67+/-0.32 from 0.14+/-0.17 at initial. Six eyes (37.5%) had complicated disorders at early postoperative period and 5 eyes (31.25%) had at their final visits. No exposure of suture material over the conjunctiva did occur. CONCLUSIONS: These results suggest that translocated suture to superotemporal sclera may be an efficient method for reducing exposure rate of suture material after TSFPCIOL.

Evaluation of Indocyanine Green Influence on Cultured an Retinal Pigment Epithelial Cells

PURPOSE: To evaluate how much the indocyanine green (ICG) influence cultured human retinal pigment epithelial (RPE) cells(ARPE-19; ATCC No. CRL-2302). METHODS: Human RPE cells were incubated with media and various concentration of ICG. Their viability upon different ICG concentration and exposure time were examined by MTT assay. Also, the amount of ICG to be influxed onto the cells was estimated with the flow cytometry and the confocal laser scanning microscopy. RESULTS: The viability examined by MTT assay decreased upon different ICG concentration and exposure time. Also, they were effected in the condition of very low dose of ICG (0.00016%, 0.0008%). The amount of ICG to be influxed onto cells increased upon its concentration and time to be exposed. CONCLUSIONS: In the exposure of RPE cells to ICG, it accumulated in the cells and their viability decrease. These results should be taken into account when ICG is used for macular hole surgery.