Correlation between CD44 Variants Expression, Microvessel Density and VEGF Expression and HPV 16/18 Subtypes in Squamous Neoplasia of the Uterine Cervix / 대한부인종양콜포스코피학회잡지

OBJECTIVE: In the development of squamous neoplasia of the uterine cervix, high risk HPV infection has been followed by CD44 variant expression and angiogenesis. The aim of this study is to evaluate the sequential changes and relatianship of CD44 variant expression, microvessel density (MVD), and VEGF expression in CIN III, microinvasive (MI) and invasive (CA) squamous cell carcinoma. METHODS: The materials were 17 cases of CIN III, 14 cases of MI and 15 cases of CA. In situ PCR for HPV 16/18 and immunohistochemical studies for CD44std, v6, v7/8, CD 31 for MVD, and VEGF were performed. RESULTS: CD44std expression was decreased in squarnous neoplasia compared to normal, and CD44v6 and v7/8 expressions were increased, however, there was no statistical significance. Accentuated staining of CD44v6 and v7/8 along the infiltrating borders was noted in 76.9% and 69.2% of MI and in 71.4% and 42.9% of CA, respectively. High MVD and VEGF 2+ expression were higher in the squamous neoplasia compared to narmal, however, there was no significant difference between the squamous neoplasia and no significant relationship between MVD and VEGF expression. The expression rates of CD44std and CD44v6 were higher in HPV 16/18 negative squamous neoplasia and that of CD44v7/8 was higher in HPV 16/18 pasitive squamous neoplasia, however, their differences were not significant. The incidences of high MVD and VEGF 2+ expression were higher in HPV 16/18 positive suqmaous neoplasia with no statistical significance. CONCLUSIONS: These results suggest that CD44 variants expression and angiogenesis are involved in the development of squamous neoplasia of the uterine cervix, however, HPV 16/18 might not be related to CD44 variant expression and angiogenesis.

A Case of Ectopic Hydatidiform Mole in the Uterine Cornua / 대한산부인과학회잡지

Ectopic pregnancy refers to gestation in which the fertilized ovum implants on any other tissure than tbe mucus membrane lining the uterine cavity. Cornual (intertitial) pegnancy is a relatively uncommon but potentially catastrophic form of ectopic pregnancy, defined as implantation in the intramural portion of a fallopian tube. A hydatidiform mole usually occupies the uterine cavity but it's occurrence in the uterine tube is extremely rare. Only a few cases of primary hydatidifm mole of the tube have been reported. We experienced a case of ectopic hydatidiform mole in the uterine cornua and so present it with brief review of literatures.

A Case of Cervical Endometriosis with Cystic Change / 대한산부인과학회잡지

Cervical endonetriosis with cystic change is extremely rare lesion. We experienced onecase of cervical endometriosis in 33 year old woman, who had no symptoms, so we reprot thecase and brief review of literatures.

Plasma Fibronectin Levels in Preeclampsia / 대한주산의학회잡지

Current concept of the pathogenesis of preeclampsia involves the generalized dysfunction of maternal vascular endothelial cells. Using ELISA (enzyme-linked immunosorbent assay) method, the fibronectin, as a marker of endothelial cell injury, was measured in the group of 35 cases of preeclampsia (mild; 15 and severe; 20 cases) and the control group of 21 cases of normal pregnancy. The clinical profiles, hematologic and renal function test results were compared and correlated with the levels of plasma fibronectin between two groups. The results obtained were as follows 1. The mean age between preeclampsia and control group was not different statistically, but gestational age, birth weight and maternal weight were different significantly between two groups. 2. Compared with normal control group, plasma levels of fibronectin were signifi- cantly elevated in preeclampsia group. As to the differential validity between two groups (control vs preeclampsia), using cutoff value of 380 mg/dl, sensitivity was 95.7 %, specificity was 90.5 %, predictive value was 93.8 %. As to the differential validity between mild and severe preeclampsia, using cutoff value of 742 mg/dl, sensitivity was 65.0 %, specificity was 73.3 %, predictive value was 76.5%. 3. As to the correlationship between plasma fibronectin levels and hematologic test results, uric acid and creatinine levels showed positive correlation in preeclampsia. Based upon these study results, plasma fibronectin could be a useful parameter for the differential diagnostic validity and grade of preeclampsia.