ABSTRACT
Introduction: Clindamycin has been used to treat pneumoniaand soft tissue and musculoskeletal infections due to MRSA.One important problem in Clindamycin treatment is the riskof clinical failure during therapy caused by MLSB inducibleresistance. The Clinical and Laboratory Standards Institute(CLSI) suggest D-test, which is a phenotypic showingtechnique for inducible Clindamycin resistance.Material and Methods: We analyzed antimicrobialsusceptibility testing by Kirby Bauer disk diffusion method.Methicillin resistance was detected with cefoxitin (30 µg) diskand inducible clindamycin resistance was unwavering in allerythromycin resistant isolate by using D-zone test.Results: 100 S. aureus isolate 37 (36.6%) were methicillinresistant (MRSA) and 63 (63.4%) were methicillin-sensitiveS. aureus (MSSA). Although, mainstream of the MRSAisolates were imitative from pus samples 15, however, the S.aureus isolates imitative from post-operative wound infectionwere mainly MRSA 7. A total of 21 S. aureus isolates withiMLSB phenotype shown that they were 100% susceptible tovancomycin and linezolid, with modest sensitivity (71.14%) togentamicin, cefuroxime and slightest sensitivity to (23.81%)doxycycline, (20.95%) ciprofloxacin.Conclusion: Outstanding to high happening of erythromycinresistance amongst S. aureus isolates, we recommend thatD-zone test have to be regularly done in all laboratories forsuitable recommendation of clindamycin and thus preventingappearance of inducible resistant strains and managementfailure.
ABSTRACT
Introduction: Surgical site infections are most commonhospitals acquired infections and are an important cause ofmorbidity and mortality. The objective of our study is toconclude the causative bacteria and antimicrobial sensitivityof surgical site infections.Material and methods: A total of 275 various clinicalsamples received in Microbiology Laboratory, GovernmentMedical College, Bettiah (West Champaran) Bihar andAssociated Hospital. from March 2018 to April 2019. Atotal 101 Staphylococcus aureus isolated, were identifiedby standard biochemical methods. Antibiotic susceptibilitytesting was performed by Kirby Bauer Disc Diffusion method.Methicillin resistance was detected by using cefoxitin (30µg)disc diffusion method as per CLSI guidelines 2016.Result: Out of the 275 aerobic bacteria which were isolated,144 were gram positive cocci (52.37%) and 131 were gramnegative bacilli (47.63%). The most common pathogenfollowed by Staphylococcus aureus 101 (36.36%). Otherorganisms were Escherichia, Pseudomonas, Klebsiella,Citrobacter, Proteus, and Enterococcus. The Antimicrobialprofile of 101 Staphylococcus aureus isolates among MRSA,resistance those they were 100% sensitive to linezolidand vancomycin, with moderate sensitivity (71.14%) tocefuroxime, gentamicin and least sensitivity to (23.81%)doxycycline, (20.95%) ciprofloxacin.Conclusion: Isolation of MRSA patients and carriers in thehospitals, regular surveillance, and monitoring of antibioticsusceptibility pattern of the hospital and community of thatregion regularly and formulation of antibiotic policy may helpin reducing the treatment failures.