ABSTRACT
OBJECTIVE: To investigate the effects of GnRH agonist and antagonist protocols on preimplantation genetic testing outcomes of chromosomal translocation carriers.METHODS: The clinical data of 226 patients with chromosomal translocation were analyzed retrospectively between Jan. 2015 and Dec. 2017 in Shengjing Hospital Affiliated to China Medical University.The patients were divided into GnRH agonist group(174 cases)and GnRH antagonist group(52 cases),then the duration of gonadotropin stimulation,dosage of gonadotropin used,embryonic quality,normal and balanced embryo numbers,per transfer cyclepregnancy rate,abortion rate and continuous pregnancy rate and accumulated pregnancy rate were analyzed.RESULTS: There were no statistical differences in the number of retrieved oocytes,number of MⅡ oocytes,fertilization rate,cleavage rate,blastocyst formation rate,number of high-quality embryo,number of blastocyst biopsy,normal and balanced embryo numbers,per transfer cycle pregnancy rate,abortion rate,continuous pregnancy rate or accumulated pregnancy rate between GnRH agonist group and GnRH antagonist group(P>0.05).The duration of gonadotropin stimulation(9 d vs. 10 d)and dosage of gonadotropin(2100 U vs. 2400 U)used in the GnRH antagonist group were significantly less than the GnRH agonist group(P<0.05).CONCLUSION: There are no significant differences in embryo quality or pregnancy rate between the GnRH agonist group and antagonist group during preimplantation genetic testing,but the GnRH antagonist group have an advantage in Gn duration and dosage.
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BACKGROUND: Embryonic stem cells are pluripotent stem cells that can differentiate into all cell types and propagate themselves indefinitely in vitro, which have been used widely in biological research. OBJECTIVE: To summarize the culture methods of embryonic stem cells in vitro, as well as the significance, methods, clinical applications of embryonic stem cells as genetic disease models. METHODS: Relevant articles were searched in PubMed using the key words of "embryonic stem cell, genetic diseases" which appeared in the titles and abstracts. The latest articles were preferred. Totally 43 eligible articles were obtained for result analysis. RESULTS AND CONCLUSION: In the genetics study, embryonic stem cells have been widely used to construct monogenic and chromosome disease models, which can make up the limitations of animal and cell models. Models constructed by embryonic stem cells are conducive to the investigations on pathological processes and molecular mechanisms of genetic diseases. But there are also some ethic disputes on the use of embryonic stem cells, which need further studies.
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Bushen Zhuyun Decoction (BZD) on the reproductive capacity of mice.</p><p><b>METHODS</b>Totally 60 female Kunming mice were randomly divided into the experimental group and the control group. BZD was perfused by gastric tube to mice in the experimental group, twice daily. Meanwhile, mice in the control group were administered with normal saline by gastrogavage. Mice were sacrificed on the 10th day and the 20th day of medication respectively, 15 mice each time in each group. The serum levels of estradiol and progesterone were detected by radioimmunoassay. The morphological changes of uterus and ovary were observed using HE staining. The expressions of leukaemia inhibitory factor (LIF), epidermal growth factor (EGF), and calcitionin (CT) were determined using immunohistochemical assay.</p><p><b>RESULTS</b>Compared with the control group at the same time point, the weights of uterus and ovary increased, the serum levels of estradiol and progestogen increased, the expressions of LIF, CT, and EGF increased in the experimental group on the 10th day and the 20th day of medication (P <0.05, P <0.01). Better results were shown on the 20th day of medication (P <0.05). Histological results showed increased ovarian follicle numbers, increased endometrial gland numbers, endometrial hyperplasia (stratiform arranged), and increased stromal cells in the experimental group, especially on the 20th day of medication.</p><p><b>CONCLUSION</b>BZD could improve the reproductive capacity by advancing the development of generative organs, promoting the secretion of estradiol and progestogen and the follicular growth, and increasing the receptivity of endometrium.</p>