ABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinical features, early diagnosis and surgical treatment of the hand glomus tumor in the female.</p><p><b>METHODS</b>Thirty female patients with the hand glomus tumor underwent early resection by microsurgery. The average age was 35 years ranging from 18 to 48 years. The course of disease was from 3 months to 3 years. All patients' tumors occured on single finger.</p><p><b>RESULTS</b>In the 30 cases, there were 28 patients with localized eminences and hepatic plaques under the nail. The compression of distal phalanx could be seen on radiographs in 18 patients, definite and localised tenderness on nails could be found in all cases, the tenderness disappeared and no recurrence after 15 months of follow-up in all cases.</p><p><b>CONCLUSION</b>The hepatic plaque under the nail and fixed tenderness point on the nail are of significance in diagnosis for the hand glomus tumor in the female, the early microsurgical resection is unique effective treatment to this disease.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Middle Aged , Early Diagnosis , Fingers , General Surgery , Glomus Tumor , Diagnosis , General Surgery , Nail Diseases , Diagnosis , General SurgeryABSTRACT
<p><b>BACKGROUND</b>Histone deacetylase inhibitors (HDACIs) have been reported to induce apoptosis in cancer cells. The effects of trichostatin A (TSA) on gastric cancer cells have not been well characterized. This study was aimed to explore the effects and mechanisms of TSA on human gastric cancer SGC-7901 cells.</p><p><b>METHODS</b>The cells were treated with TSA and analyzed by cell proliferation assay, Western blot, TUNEL assay, flow cytometry by fluorescein isothiocyanate (FITC) conjugated with Annexin V and PI staining, immunofluorescence analysis, analysis of subcellular fractionation, gene chips and real time polymerase chain reaction (PCR).</p><p><b>RESULTS</b>TSA could inhibit cell growth and induced apoptosis in gastric cancer SGC-7901 cells through the regulation of apoptosis-related genes, such as Bcl-2, Bax and survivin. Further study indicated that the pan-caspase inhibitor z-VAD-fmk did not inhibit the apoptosis induced by TSA, and we did not observe the cleavage of poly ADP ribose polymerase (PARP) after TSA treatment too. In addition, apoptosis inducing factor (AIF) and EndoG were found to translocate from mitochondria to nucleus in the immunofluorescence assay and the Western analysis of subcellular fractionation confirmed the result of immunofluorescence assay.</p><p><b>CONCLUSIONS</b>The apoptosis induced by TSA in gastric cancer SGC-7901 cells involves a caspase-independent pathway.</p>