ABSTRACT
Abstract We sought to compare the characteristics and clinical significance of neutrophil extracellular traps in gingival samples from patients with periodontitis and those with gingivitis. The clinical indexes of gingival samples from patients with periodontitis and gingivitis were measured; the expression of TNF-alpha and IL-8 was measured by real-time fluorescent quantitative PCR; and the expression of TLR-8 and MMP-9 was measured by western blotting assays. Chemotaxis, phagocytosis and phagocytic activity of neutrophils were measured. Compared with the healthy group, the expression of TNF-α and IL-8 in the periodontitis group and the gingivitis group increased significantly (p < 0.05), and TNF-α in the gingivitis group was significantly lower than that in the healthy group (p < 0.05). The expression of IL-8 in the periodontitis group was significantly higher than that in the periodontitis group (p < 0.05). Furthermore, the expression of TLR-8 and MMP-9 in the periodontitis group was different from that in the gingivitis group and the healthy group, and the expression of TLR-8 and MMP-9 in the gingivitis group was significantly different from that in the healthy group (p < 0.05). In addition, the neutrophil mobility index in healthy people was 3.02 ± 0.53, that in the periodontitis group was 2.21 ± 0.13, and that in the gingivitis group was 2.31 ± 0.12. In conclusion, the chemotaxis of neutrophils in gingival samples of patients with periodontitis and gingivitis was decreased, the phagocytotic ability and activity of neutrophils were reduced, and the release of the extracellular trap-releasing inducible factors TNF-alpha and IL-8 also declined.
Subject(s)
Humans , Male , Female , Young Adult , Periodontitis/pathology , Extracellular Traps , Gingivitis/pathology , Neutrophils/pathology , Reference Values , RNA/analysis , Case-Control Studies , Periodontal Index , Blotting, Western , Interleukin-8/analysis , Actins/analysis , Tumor Necrosis Factor-alpha/analysis , Matrix Metalloproteinase 9/analysis , Electrophoresis, Agar Gel , Toll-Like Receptor 8/analysis , Real-Time Polymerase Chain Reaction , Middle AgedABSTRACT
OBJECTIVE@#The study was carried out on time limit of ACP and sperm positive-detected in mixed spot.@*METHODS@#600 vaginal swabs from living body after sexual intercourse were tested by routine and three-blindness methods.@*RESULTS@#The longest time limit of ACP which was positively detected is 255 h, average time limit is 52 h, 41.3% of those samples was ACP-detected in positive degree (++). The longest time limit of sperm positively detected is 132 h, average time limit is 29 h. The number of sperms observed in 34.3% of those samples with positive result, is from 6 to 10/HP.@*CONCLUSION@#This study is helpful to forensic identification of sex cases.