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Objective: Integrating drug-likeness and network targets to preliminarily predict and explore the targets and pathways of Danggui Buxue Jiawei decoction(DBJD)in the prevention and treatment of diabetic retinopathy(DR). Methods: The chemical constituents of DBJD were identified by UPLC-Q-TOF/HRMS E analytical techniques. The related websites were used to esti mate the drug-likeness for all identified constituents. Then, for the selected drug-like constituents, targets-predicting was carried out on PharmMapper, and the targets for DR were searched by CTD, TTD and GeneCards. The component-target and disease-target net works were integrated to establish a large network, and core targets were searched on the basis of the DC, CC and BC values of the large network. Moreover, indirect targets were further searched on the basis of the core targets. Then, an all potential targets-related network was constructed and related key targets were searched by the constructed network. The key targets were verified by docking with the related components. Gene ontology(GO)biological analysis, Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis and Reactome pathway analysis were carried out on all potential targets. Eventually, a large Prescription-Medicinal material-Component-Direct target-Indirect target-Pathway integrated network was established for the DBJD studies. Results: A total of 55 chemical constituents were identified by UPLC-Q-TOF/HRMS E. Among them, 33 components were selected by the drug-likeness esti mation. The core targets consisted of 36 targets, and 20 indirect targets were obtained by relating with the core targets. By comparing de gree values, 6 key targets were established: AKT1, MAPK3, VEGFA, INS, MAPK1 and CASP3. In molecular docking tests, these key targets showed good binding activities with tanshinone, salvianolic acid B, ginsenoside Rg 1, notoginsenoside R 1, tanshinone Ⅱ A and other quality control components. Pathway analysis suggested that DBJD could likely interfere with the diabetic retinopathy by regulating oxidative stress, inflammatory factors and sugar metabolism. Conclusion: Accurate prediction of the action mechanism of complex Chinese medicinal ingredients based on the drug-likeness and network targets may be helpful to improve the blindness in relat ed research and accelerate the research process in modernization of traditional Chinese medicine. In this study, the molecular mecha nism of DBJD for the prevention and treatment of DR is preliminarily investigated and discussed, which could provide a valuable refer ence for future in-depth study.
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<p><b>OBJECTIVE</b>To explore whether PCDH15-SI is stable and accurate to diagnose NK/T-cell lymphoma or not.</p><p><b>METHODS</b>The paraffin-biopsiy specimens were collected from 45 cases of NK/T-cell lymphoma and 33 cases of non-NK/T-cell lymphoma in Department of pathology, West China Hospital of Sichuan University. The paraffin sections were stained by immunohistochemistry and blank controls were set up. The peripheral blood was collected from these cases of NK/T-cell lymphoma and non-NK/T-cell lymphoma, as well as from the normal controls in the same hospital. The serum PCDH15 levels were detected by enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>Immunohistochemistry showed that PCDH15 were expressed in biopsy specimens of both the NK/T-cell lymphoma in the experimental group and the non-NK/T cell lymphoma in the control group. The expression rate in the NK/T-cell lymphoma group was higher than that in control group. The PCDH15-SI can be detected in NK/T cell lymphoma, non-NK/T cell lymphoma and the normal controls, which is higher in lymphoma patients than that in control, and the serum level was higher in the non-NK/T-cell lymphoma patients than that in the NK/T-cell lymphoma patients. Also, the serum level was higher in the patients with advanced disease than that in the patients at early stage.</p><p><b>CONCLUSION</b>PCDH15 does not act as the new diagnostic marker for NK/T cell lymphoma. The serum level of PCDH15-SI may be helpful to the staging and judging therapeutic effect and prognosis for the patients with non-Hodgkin's lymphoma.</p>
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<p><b>OBJECTIVE</b>To explore the effect of recombinant human erythropoietin (rhEPO) on myeloma cell line MPC-11 in vitro and its anti-tumor mechamism so as to provide the theoretic evidence for treatment of multiple myeloma by using rhEPO.</p><p><b>METHODS</b>MPC-11 cells were cultured in RPMI 1640 medium, then the MPC-11 cells in logarithmic growth phase were seed in 96 well culture plates at a density of 1×10/ml cells with 100 µl per well. For all the experiments, the control and rhEPO groups were set up, and the rhEPO group was divided into different concentration groups: 20, 40, 60, 80, 100, 200 and 400 rhEPO U/ml. The cultured MPC-11 cells in each groups were collected at day 1, 2, 3, 4, 5 and 6, and the viability of MPC-11 cells was assayed by using CCK-8 method, the MPC-11 cell apoptosis was detected by flow cytometry with Annexin V/PI double staining, the levels of IL-6, IgG and kappa light chain in cell supernatant were detected by ILISA.</p><p><b>RESULTS</b>The apoptosis rate in MPC-11 cells treated with 200 U/ml of rhEPO at day 21 was significantly higher than that in control group(P<0.05); the apoptosis rate in MPC-11 cells treated with 400 U/ml of rhEPO at day 10, 15, 21 was higher than that in control group at same time points, respectively (P<0.05), suggesting that the MPC-11 cell apoptosis rate was enhanced with increase of rhEPO concentration and prolonging of culture time(P<0.05), the IL-6 level in supernatant of cell treated with 400 U/ml of rhEOP for 15 days was lower than that in control group(P<0.05), while the IL-6 level in supernatant of cells treated with 100, 200, 400 U/ml of rhEPO for 21 days decreased (P<0.05); The levels of IgG and kappa light chain in supernatant of cells treated with 400 U/ml of rhEPO for 21 days all were significantly lower than those in control group(P<0.05).</p><p><b>CONCLUSION</b>The rhEPO can induce apoptosis of MPC-11 cells with concentration- and time-dependent manner, and can reduce levels of IgG, kappa light chain secreted by MPC-11 cells with time-dependent manner.</p>
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<p><b>BACKGROUND</b>The dilemma of pathogens identification in patients with unidentified clinical symptoms such as fever of unknown origin exists, which not only poses a challenge to both the diagnostic and therapeutic process by itself, but also to expert physicians.</p><p><b>METHODS</b>In this report, we have attempted to increase the awareness of unidentified pathogens by developing a method to investigate hitherto unidentified infectious pathogens based on unbiased high-throughput sequencing.</p><p><b>RESULTS</b>Our observations show that this method supplements current diagnostic technology that predominantly relies on information derived five cases from the intensive care unit. This methodological approach detects viruses and corrects the incidence of false positive detection rates of pathogens in a much shorter period. Through our method is followed by polymerase chain reaction validation, we could identify infection with Epstein-Barr virus, and in another case, we could identify infection with Streptococcus viridians based on the culture, which was false positive.</p><p><b>CONCLUSIONS</b>This technology is a promising approach to revolutionize rapid diagnosis of infectious pathogens and to guide therapy that might result in the improvement of personalized medicine.</p>
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Female , Humans , Male , Herpesvirus 4, Human , Genetics , High-Throughput Nucleotide Sequencing , Methods , Viridans Streptococci , GeneticsABSTRACT
OBJECTIVE@#To discuss the influencing factors of using late postmortem phenomena to estimate PMI and to provide experience for an accurate estimation.@*METHODS@#Forty-nine corpses of late postmortem were collected in Shaoxing City, Zhuji area from 2004 to 2011. The related factors were analyzed including season, scene, estimated PMI, exact PMI, cause of death and main factors effected PMI, etc.@*RESULTS@#Of all 49 cases, 20 corpses were outdoor, 11 were indoor and 18 were in water. Thirty-seven cases were successful to estimate PMI and 12 cases were unsuccessful. The main factors affected PMI were infection, poisoning, human destruction and high-pressure electric shock, etc.@*CONCLUSION@#In general, PMI can be correctly estimated by late postmortem phenomenon. When the cases included infection, poisoning and human destruction, we should estimate PMI with the comprehensive analysis.