ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulation of Cha Gan Beng Ga on the activity of biomarker PGC-1α in vivo and in vitro, and lay the foundation for studying the efficacy result of Cha Gan Beng Ga on xenograft tumor model and extracting active constituents.</p><p><b>METHOD</b>(1) The coarse powder of Cha Gan Beng Ga was extracted with 70% ethanol solution through heating and refluxing, and finally was used to freeze dry powder. (2) 50 mg x kg(-1) of freeze-dried power was orally administrated to KM and C57BL/6J mice once daily, lasting for 5 consecutive days; different concentrations of extracted materials was given to non-small cell lung cells A549. (3) The expression level of PGC-1α mRNA was quantitatively determined in lung tissue of mice and non-small cell lung cells A549.</p><p><b>RESULT</b>The expression levels of PGC-1α in lung tissue of different mice strains had an increasing tendency. Furthermore, the expression levels of PGC-1α in non-small cell lung cells A549 also had an increasing tendency, showing dose and time-dependent relationships.</p><p><b>CONCLUSION</b>Mongolian Medicine Cha Gan Beng Ga could induce the over-expression of PGC-1α mRNA in lung tissue of mice and in non-small cell lung cells A549. The present results will lay foundation for studying the efficacy result of antitumor and active constitutes in future.</p>
Subject(s)
Animals , Humans , Male , Aconitum , Chemistry , Biomarkers, Tumor , Genetics , Carcinoma, Non-Small-Cell Lung , Genetics , Pathology , Cell Line, Tumor , Dose-Response Relationship, Drug , Gene Expression Regulation, Neoplastic , Lung , Metabolism , Lung Neoplasms , Genetics , Pathology , Medicine, Mongolian Traditional , Mice, Inbred C57BL , Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha , Plant Extracts , Pharmacology , Reverse Transcriptase Polymerase Chain Reaction , Time Factors , Transcription Factors , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To study the influence of several excipients on damp-proof performance of pharmaceutical materials of traditional Chinese medicine.</p><p><b>METHOD</b>The moisture absorption rate and parameters of hydroscopicity were used as the evaluation index of the damp-proof property of the complex Chinese medicine and preparation 1 and 2.</p><p><b>RESULT</b>The moisture rate of complex Chinese medicine 1 was 62.54%, the critical relative humidity (CRH) was 38%. The moisture rate of complex Chinese medicine 2 was 16.36%, the CRH was 53%. Excipients had different effect on lower the hyproscopic property of complex Chinese medicine 1 and 2. The initial moisture adsorption velocity of excipients of complex Chinese medicine 1 in a ascending order were dextrin < calcium hydrogen phosphate < micro crystalline cellulose < lactose < ethyl cellulose < mannitol < hydroxypropyl methyl cellulose. The moisture adsorption acceleration of excipients in a ascending order were dextrin = calcium hydrogen phosphate = micro crystalline cellulose < mannitol = ethyl cellulose = lactose < hydroxypropyl methyl cellulose. The moisture adsorption rate of excipients in a ascending order were dextrin < lactose < calcium hydrogen phosphate < ethyl cellulose < micro crystalline cellulose < mannitol < hydroxypropyl methyl cellulose. The initial moisture adsorption velocity of excipients of complex Chinese medicine 2 in a ascending order were mannitol < dextrin < calcium hydrogen phosphate < lactose < ethyl cellulose < micro crystalline cellulose < hydroxypropyl methyl cellulose . The moisture adsorption acceleration of excipients in a ascending order were mannitol = dextrin = calcium hydrogen phosphate < lactose < ethyl celluloselactose < micro crystalline cellulose < hydroxypropyl methyl cellulose. The moisture adsorption rate of excipients in a ascending order were mannitol < dextrin < calcium hydrogen phosphate < lactose < ethyl cellulose < micro crystalline cellulose < hydroxypropyl methyl cellulose.</p><p><b>CONCLUSION</b>The choosing of damp-proof excipients of preparation based on the property of the complex traditional Chinese medicine. The study provided experimental evidences for the research and development of the pharmaceutical materials of traditional Chinese medicine.</p>
Subject(s)
Absorption , Drugs, Chinese Herbal , Chemistry , Excipients , Chemistry , Humidity , Medicine, Chinese TraditionalABSTRACT
<p><b>OBJECTIVE</b>To prepare four breviscapine lipid carrier gels and general gel, and to study the transdermal promotive effects of them in vitro.</p><p><b>METHOD</b>Improved Franz-type diffusion cell and isolated rat skin were used as transdermal barrier, and the transdermal property was fitted by pharmacokinetics equations. The unit area cumulative permeation quantity of breviscapine was determined by HPLC method.</p><p><b>RESULT</b>The 24 hours' unit area cumulative permeation quantity of SLN gel was the highest, followed in descending order were transfersomal gel, liposomal gel, NLC gel and general gel. The transdermal property in vitro of SLN gel fitted in with Higuchi pharmacokinetics equation.</p><p><b>CONCLUSION</b>The lipid carrier gels had different transdermal promotive effects to breviscapine, SLN gel was the best one as breviscapine transdermal carrier.</p>