Determination of gossypol in cotton root bark by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a method for the determination of gossypol in cotton root bark.</p><p><b>METHOD</b>Samples were extracted with acetone, and determined on a C18 column with the mobile phase (Acetonitrile-0.2% phosphoric acid, 85:15) and UV-235 nm detector.</p><p><b>RESULT</b>The recovery rate was 97.6%, RSD 1.59% (n = 5).</p><p><b>CONCLUSION</b>This method can be used for the determination of gossypol in cotton root bark and the content of gossypol in three different species has been determined.</p>