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Objective: To establish a cell-based multi-parameter mitochondrial structure and functional evaluation system for hydrogen peroxide (H2O2) injury in cardiomyocytes and investigate the protective effect of Qishen Yiqi Formula on H9c2 cells and its mechanism. Methods: For the in vitro myocardial cell injury model, H9c2 cells were divided into control, model (H2O2), positive control (carbonyl cyanide-cyanomethoxy basic hydrazine, 5 μmol/L), and Qishen Yiqi Formula (0.2 mg/mL) groups, with three duplicates in each group, and were cultured in triplicate for 24 h with corresponding drugs, followed by 2 h H2O2 induction. H2O2 injury model was established with H9c2 cell line. Mitochondrial function and morphological texture were evaluated by Operetta high content imaging system. Mitochondrial respiration and bioenergetic metabolism states were measured by Seahorse Bioscience XF extracellular flux analyzer. Cardiomyocyte apoptosis were quantified by flow cytometry. Results: Qishen Yiqi Formula prevented the decrease of mitochondrial membrane potential and the increase of mitochondrial mass and improved mitochondrial morphological integrity. Functionally, Qishen Yiqi Formula increased basic oxygen consumption rate, ATP-linked oxygen consumption rate, maximum oxygen consumption, and the reserve capacity. It also reduced the apoptotic rate at early and end stage and increased the myocyte survival. Conclusion: Based on the structure and functional evaluation of the mitochondria, results indicated that the compound Qishen Yiqi Formula protected the H9c2 cells by improving the mitochondrial function and energy metabolism as well as reducing the apoptosis.
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Objective To explore the therapeutic effects of Baizhu Huanglian Decoction on rats with ulcerative colitis (UC) induced by trinitrobenzene sulfonic acid (TNBS). Methods SD rats were used to make rat UC model by TNBS enema. A total of 74 rats were divided into 6 groups with half of each group being male. Model group had 14 rats, and Baizhu Huanglian Decoction low-, middle- and high-dose groups, control group and sulfasalazine group had 12 rats in each group. One rat in the model group and one in the sulfasalazine group were dead on the 2nd day of modeling. After modeling, the rats of Baizhu Huanglian Decoction low-, middle- and high-dose group were intragastrically given (2 times a day, each time with 2 mL) Baizhu Huanglian Decoction of 6.895, 13.790 and 27.580 g/kg, respectively. Sulfasalazine group was given sulfasalazine suspension by gavage 0.2 g/kg (2 times a day, each time with 2 mL) and model group was given the same amount of normal saline after modeling. Normal control group was always given normal saline. After 10 days of continuous administration, the body mass and fecal characteristics of the rats were observed. At the end of the administration, the colonic pathological changes of colon and the length of colorectal were measured. The colon pathology and colon mucosal injury index (CMDI) score were evaluated. The levels of nitric oxide (NO), inducible nitric oxide synthase (iNOS), myeloperoxidase (MPO) and interleukin-6 (IL-6) were measured by ELISA. Results Compared with the model group, the body mass of the rats were significantly increased on the 4th-10th day after administration in the Baizhu Huanliang Decoction low-, middle- and high-dose groups and sulfasalazine group (all P0.05), which had no significant difference versus normal control group. Fecal characteristic scores of the Baizhu Huanglian Decoction high-dose groups and the sulfasalazine group were lower than those of the model group and the Baizhu Huanglian Decoction middle- and low-dose groups, but the differences were not statistically significant. Compared with the model group, the colorectal lengths of the rats were significantly longer in Baizhu Huanglian Decoction middle- and high-dose groups and sulfasalzine group (all P0.05). The colon pathology scores were significantly lower in the Baizhu Huanglian Decoction low-, middle- and high-dose group, and sulfasalazine group versus the model group (all P0.05). CMDI scores of Baizhu Huanglian Decoction high-dose group and sulfasalazine group were significantly lower than those of the model group (both P0.05). The levels of NO, iNOS, MPO and IL-6 of the rat colonic tissues were significantly lower in Baizhu Huanliang Decoction low-, middle- and high-dose groups, and sulfasalazine group than those in the model group (all P0.05). Conclusion Baizhu Huanglian Decoction can improve the general condition and colonic lesions of rats with UC, decreasing the levels of NO, iNOS, MPO and IL-6 and reducing the inflammation.
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METHODS: Male Sprague Dawley rats which were used lor Langendoff isolated heart perfusion were divided into four groups; normal control group (n=6), 120 group (n=1), 130 group (n=8) and 140 group (n=8), these hearts were subjected to global ischemia for 20, 30 and 40 min respectively. Then coronary flow, heart rate, creatinine kinase and lactate dehydrogenase in effluent and the changes of cardiac function parameters were measured in different groups. Infarct and risk areas were measured by planimetry using Image/J software.
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<p><b>BACKGROUND</b>No-reflow phenomenon during percutaneous coronary intervention (PCI) for acute myocardial infarction (AMI) is a predictive factor of continuous myocardial ischemia, ventricular remodeling and cardiac dysfunction, which is closely associated with a worse prognosis. This study aimed to evaluate intracoronary nitroprusside in the prevention of the no-reflow phenomenon in AMI.</p><p><b>METHODS</b>Ninety-two consecutive patients with AMI, who underwent primary PCI within 12 hours of onset, were randomly assigned to 2 groups: intracoronary administration of nitroprusside (group A, n = 46), intracoronary administration of nitroglycerin (group B, n = 46). The angiographic results were observed. The real-time myocardial contrast echocardiography (RT-MCE), including contrast score index (CSI), wall motion score index (WMSI), transmural contrast defect length (CDL) and serious WM abnormal length (WML) were recorded at 24 hours and 1 week post-PCI. High sensitivity C-reactive protein (Hs-CRP) was examined by immune rate nephelometry. N-terminal prohormone brain natriuretic peptide (NT-proBNP) was tested with enzyme-linked immunosorbent assay. Patients were followed up for six months. Major adverse cardiac events (MACE) were recorded.</p><p><b>RESULTS</b>The incidence of final TIMI-3 flow in group A was much higher than that in Group B (P < 0.05), final corrected TIMI frame count (cTFC) in group A decreased significantly than that in group B (P < 0.01). The CSI, CDL/LV length, WMSI and WL/LV length in group A were significantly lower than that in group B (P < 0.01). Levels of Hs-CRP and NT-proBNP at 1 week post-PCI decreased significantly in group A than that in group B (P < 0.01). Patients were followed up for 6 months and the incidence of MACE in group A was significantly lower than that in group B (P < 0.05).</p><p><b>CONCLUSION</b>Intracoronary nitroprusside can improve myocardial microcirculation, leading to the decrease of the incidence of no-reflow phenomenon and better prognosis.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Acute Disease , Angioplasty, Balloon, Coronary , C-Reactive Protein , Coronary Angiography , Coronary Circulation , Follow-Up Studies , Myocardial Infarction , Blood , Therapeutics , Natriuretic Peptide, Brain , Blood , Nitroprusside , Peptide Fragments , BloodABSTRACT
<p><b>OBJECTIVE</b>To evaluate the feasibility of employing metabonomics method in identification of oral pathogenic bacteria.</p><p><b>METHODS</b>The Streptococcus mutans ATCC25175 and Actinomyces viscosus ATCC15987 were respectively inoculated in same certain culture medium. The growth curves of the inoculated bacteria were drown by turbidimetry. The culture solutions in four different growth phases of the both bacteria were used to test with the 1H-Nuclear magnetic resonance (1H-NMR) spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).</p><p><b>RESULTS</b>The PCA showed the obvious clustering phenomena and the points of two group data stayed differentially together by two clusters. Therefore, the NMR-based metabonomics profiles can discriminate the two different kind of bacteria.</p><p><b>CONCLUSION</b>The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria.</p>