ABSTRACT
<p><b>OBJECTIVE</b>To test anti-Sp17 antibodies in the serum of AsAb positive infertile patients, to investigate the proportion of anti-Spl7 antibodies in AsAb and their potential application to the serologic diagnosis of immune infertility and immunocontraception.</p><p><b>METHODS</b>With human recombinant Sp17 as the antigen, the ELISA method was used to detect the positive rate, antibody titre and content of anti-Sp17 antibodies in the AsAb positive serum.</p><p><b>RESULTS</b>The positive rate of anti-Sp17 antibodies in the AsAb positive serum was 56.5%, with no significant difference in the gender aspect. The percentage of anti-Sp17 antibodies in AsAb was (10.09 +/-7.45) %, with statistical significance (P <0.05).</p><p><b>CONCLUSION</b>Sp17 is an important sperm antigen. Anti-Sp17 antibodies in the serum can be taken as auxiliary diagnostic index of infertility, and Sp17 is shown to be a potential candidate immunocontraception vaccine.</p>
Subject(s)
Adult , Female , Humans , Male , Antigens, Surface , Allergy and Immunology , Autoantibodies , Blood , Carrier Proteins , Allergy and Immunology , Contraception, Immunologic , Infertility, Male , Blood , Allergy and Immunology , Spermatozoa , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To explore the clinical significance of HBV large protein (HBV-LP) in diagnosing viral replication, we detected the HBV-LP, HBV DNA and the hepatitis B viral markers (HBV M) in the serum of the patients infected with HBV.</p><p><b>METHODS</b>HBV-LP and HBV M were analyzed by using ELISA. HBV-DNA was quantitatively detected using real-time fluorescent polymerase chain reaction.</p><p><b>RESULTS</b>(1) No significant difference between the detectable rate of HBV DNA and HBV-LP was found in the same HBV M (P 0.05). (2) No significant difference between the positive rate of HBV DNA and HBV-LP was found in HBeAg negative patients. (3) The contents of serum HBV-LP was positively correlated with the number of HBV DNA copies.</p><p><b>CONCLUSION</b>There was a close correlation between the positive rate of HBV-LP and HBV DNA, and HBV-LP is a reliable serological marker that can reflect the replication of HBV.</p>