ABSTRACT
Objective:To statistics and analyze the detection rate of 5 kinds of common respiratory virus specific IgM in children with respiratory tract infection to guide the clinical.Methods: 1 609 cases of children with respiratory tract infection in department of Pediatrics in our hospital were collected in 2012 from 1 to 12 months.Serum was collected to detect the specific IgM of respiratory syncytial virus (RSV),adenovirus (ADV),influenza A virus (IVA),influenza B virus (IVB) and parainfluenza virus ( PIV) by method of indirect immunofluorescence.Results:263 positive cases were detected from 1 609 cases ( 16.35%) , and the positive rates of IVB was the highest (8.45%) with a total of 136 cases,followed by the rest of IVA 63 cases(3.92%),PIV 62 cases (3.85%),ADV 44 cases(2.73%) and RSV 42 cases(2.61%).The positive detection rate was different in children of different age groups,and the difference was statistical significant (χ2=134.822,P<0.01).Positive detection rate was gradually increased along with the age growth,and the detection rate of school-age children was the highest (34.55%);The comparison differences of IVB ,PIV,IVA positive detection rate between different age stages were significant (χ2=102.660 ,86.145 ,39.791 ,P<0.05 ).The detection rate of IVB and IVA was the highest in school-age children,20.00%and 8.64%respectively.The detection rate of PIV was the highest (12.07%) in preschool children.The positive rates of different respiratory virus specific IgM had their own characteristics in different months .IVB appeared two positive peaks in March and October with the rate of 20.15%and 11.11%respectively;IVA appeared two positive peaks in March and May with the rate of 10.27% and 11.81% respectively;PIV and RSV , the detection rate of which was the highest ( 7.98%and 6.46 respectively ) in March increased gently from January to June ,and then declined steadily.The detection rate of ADV was the highest (6.19%) during three months of March ,April and May.Conclusion:The positive rate of 5 kinds of respiratory virus specific IgM was 16.35%,especially the detection rate of IVB was the highest.Positive detection rate of respiratory virus specific IgM was gradually increased along with the age growth;The detection rate of IVB and IVA was the highest in school-age children ,while PIV in preschool children.5 kinds of respiratory tract virus own respectively prevalent characteristics in 2012.
ABSTRACT
Objective To study whether the silica nanoparticles can be used as a biomacromolecular carrier of oral drugs.Methods Studyed the influence of the amount of calcium ions on nano silica nanoparticles morphology,the encapsulation rate of silica nanoparticles on proteins or peptides in the presence of different calcium ions,the situation of proteins or peptides released from silica nanoparticles,enzymolysis of protein packaged by silica, silica nanoparticles labeled peptides with 125 I released in mice.Results Experiments showed that the synthetic silica nanoparticles packaged with peptides could smoothly reach the small intestine through the gastric acid in mice, it had potential to be biomacromolecular carrier of oral drug. Conclusion Proteins or peptides is easy to release in alkaline condition,but release few in acidic condition of pH2 (PH of gastric acid).The silica nanoparticles prepared have the potential to be oral drug carrier.