ABSTRACT
Objective · To investigate the effect of metformin on insulin resistance (IR)-related metabolic parameters in olanzapine-bearing rats.Methods · Thirty female SD rats were randomly divided into metformin intervention group, olanzapine group and control group. During the first 6 weeks,5 mg /(kg·d) olanzapine was given to the two test groups . The control group was given the same amount of saline. From the 7th week, the intervention group began to combine with metformin 500 mg / (kg·d), while the olanzapine group combined with the same amount of saline, continuing for 4 weeks.At the end of 6th week and 10th week, intraperitoneal glucose tolerance and homeostasis model assessment IR index were assessed. Results · The area under the glucose tolerance curve (P=0.040) and the IR index (P=0.000) were significantly higher for the intervention group and the olanzapine group than the control group at the end of 6th week. At the 10th weekend, the glucose tolerance (P=0.015) and IR index in the intervention group were significantly lower than those in the olanzapine group (P=0.001). Conclusion · Metformin may rectify the impaired glucose tolerance and improve IR induced by olanzapine partly.
ABSTRACT
OBJECTIVE@#To investigate the accurate marker to detect regulatory T cells in vivo and also to evaluate the changes of CD4+ CD25+ regulatory T cells in peripheral blood from patients with papillary thyroid carcinoma and clinical significance.@*METHOD@#Forty patients with papillary thyroid carcinoma and 34 patients with thyroid adenoma were included in this study. The proportion of CD4+ D25+ CD127(low/-) regulatory T cells population and CD4+ CD25+ FoxP3- regulatory T cells population was evaluated by flow cytometric analysis.@*RESULT@#Compared with control group, the proportion of CD4+ CD25+ CD127(low/-) regulatory T cells and CD4+ CD25+ FoxP3+ regulatory T cells in PTC group increased significantly (P < 0.05). The proportions of CD4+ CD25+ CD127(low/-) regulatory T cells and CD4+ CD25+ FoxP3+ regulatory T cells were significantly higher between patients with and without cervical lymph node metastasis (P < 0.05), and extraordinarily different among patients with different clinical stages (P < 0.05).@*CONCLUSION@#CD4+ CD25+ CD127(low/-) can be used as an effective membrane marker in identification of CD4+ CD25+ regulatory T cells in stead of CD4+ CD25+ FoxP3+. The CD4+ CD25+ regulatory T cells in the peripheral blood of papillary thyroid carcinoma patients are significantly increased in comparison with that in thyroid adenoma patients. The proportions of Treg of patients with different stages, cervical lymph node metastasis are different. It may be responsible for the happen, development and recurrence of the papillary thyroid carcinoma.