Determination of Ginsenoside Rb_1 in AITONG Capsules / 浙江中医药大学学报

[Objective] To establish n HPLC method for analysis of ginsenoside Rb1 in AITONG capsules. [Method]The HPLC system consists of G1314A variable wavelength un-visible detector,Hypersil ODS(4.6mm?250mm,5?m),HP chemstation, Hp1100 Series Quaternary Pump and Vacuum degasser (G1322A). The mobile phase was: Acetonitrile:Water (30∶70,v/v). The flowrate was 1.0ml?min-1,the injection volume was 20?l,and the detector wavelength was 203nm. [Results] The method was linear at the ginsenoside Rb1 ranging from 0.04?g to 2?g. The recovery of method was 98.26%(n=5),the average RSD was 1.41% .