ABSTRACT
Objective: To study the effects of pigment epithelial-derived factor (PEDF) on the proliferation, apoptosis and invasion of squamous lung carcinoma cells in high-glucose environment so as to explore the significance of PEDF in the development, prognosis and treatment of lung cancer associated with diabetes. Methods: SK-MES-1 lung squamous carcinoma cells were cultured and divided into negative control group; high-glucose group; and PEDF+high glucose groups 1, 2 and 3. The cell morphological changes were observed under the inverted microscope. Then proliferation inhibition rates of SK-MES-1 cells in all the groups were observed by MTT assay. The cell cycle and cell apoptosis rates were detected by flow cytometry. The number of penetration cells was determined by cell invasion experiment. Expression of VEGF in culture supernatant in each group was detected by ELISA. Results: ① Compared with that in the negative control group, the proliferation inhibition rate and apoptosis rate in high-glucose group were low, the percentage of cells blocked in G0/G1 phase was decreased, the number of penetration cells was increased and the concentration of VEGF was increased (P<0.05). ② With the increase of PEDF intervention concentration, the proliferation inhibition rate and apoptosis rate in each group increased, the percentage of G0/G1 phase increased, the number of penetration cells decreased, and the concentration of VEGF decreased (P<0.05). Conclusion: ① The development of squamous cell carcinoma of the lung is promoted in high glucose. ② PEDF can inhibit the proliferation of lung squamous cell carcinoma cells in high-glucose environment, promote early apoptosis and reduce the invasiveness in the concentration-dependent manner. PEDF is predicted to be a target therapeutic drug for lung cancer complicated with diabetes mellitus.
ABSTRACT
<p><b>OBJECTIVE</b>To Study the effect of C677T and MTHFR gene polymorphism on side effects of HD-MTX in ALL children.</p><p><b>METHODS</b>The gene polymorphism of C677T A303G and MTHFR C677T were detected by PCR in 98 ALL children from January 2014 to January 2016. The side effects during HD-MTX therapy were observed, and the relationship among GSTP1, MTHFR gene polymorphism and incidence of side effect of HD-MTX were analyzed.</p><p><b>RESULTS</b>Among 98 ALL children, the gene variation was observed in 61 ALL children (62.24%). Polymorphism study on C677T A303G showed that the gene frequency of A was 84.69%, while that of G was 15.31%; for polymorphism of MTHFR C677T, gene frequency of C was 66.33%, and that of T was 33.67%. Seven patients(7.14%) experienced with bone marrow supression, 23 patients(23.47%) with liver function damage, 15 patients(15.31%) with renal function damage, 48 patients(48.98%) with gastrointestinal reactions and 46 patients(46.94%) with mucosal lesions. After adjustment of sex, age, risk stratification and dosage of MTX, the gene polymorphism had no significant relationship with bone marrow suppression, gastrointestinal reactions and mucosal lesions(P>0.05). However, the number of the mutant genes had statistically significant relationship with liver and renal function damage(P<0.05).</p><p><b>CONCLUSION</b>The risk of side effects during HD-MTX therapy increases in ALL children with combined mutation of MTHFR and C677T.</p>