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@#Objective To investigate the effect of Rhodiola Rosae P. E on the expression of c-Fos and the apoptosis of neuronal in rat brain after ischemia/reperfusion (I/R) injury. Methods 120 Wistar rats were divided into sham group, model group and intervention group,subgrouped as 3 h, 6 h, 12 h, 24 h, and 48 h. The model and intervention groups underwent middle cerebral artery occlusion, and the latter accepted Rhodiola 0.672 g/kg daily for 15 d. They were assessed with Longa's score. The expression of c-Fos and apoptosis of neuronal were measured with immunohistochemistry and TUNEL. Results The expression of c-Fos in the ischemic area increased after cerebral ischemia/reperfusion (P<0.01), peaking at 24 h after injury, and decreased in the intervention group (P<0.01), with the decrease of neuron apoptosis and Longa's score (P<0.01). Conclusion Rhodiola protects the brain tissue against ischemia/reperfusion injury by inhibiting the expression of c-Fos and apoptosis in rats.
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Objective To investigate the effect of Rhodiola Rosae P. E on the expression of c-Fos and the apoptosis of neuronal in rat brain after ischemia/reperfusion (I/R) injury. Methods 120 Wistar rats were divided into sham group, model group and intervention group, subgrouped as 3 h, 6 h, 12 h, 24 h, and 48 h. The model and intervention groups underwent middle cerebral artery occlusion, and the latter accepted Rhodiola 0.672 g/kg daily for 15 d. They were assessed with Longa's score. The expression of c-Fos and apoptosis of neuronal were measured with immunohistochemistry and TUNEL. Results The expression of c-Fos in the ischemic area increased after cerebral isch-emia/reperfusion (P<0.01), peaking at 24 h after injury, and decreased in the intervention group (P<0.01), with the decrease of neuron apop-tosis and Longa's score (P<0.01). Conclusion Rhodiola protects the brain tissue against ischemia/reperfusion injury by inhibiting the expres-sion of c-Fos and apoptosis in rats.
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Objective To evaluate the therapeutic effect of botulinum toxin type A(BTX-A)injection in the treatment of spasmodic torticollis.Methods Sixty-four patients with spasmodic torticollis underwent the treatment of intramuscular injection of BTX-A in neck muscles.As a result,the efficiency of BTX-A therapy could be evaluated successfully.Results Considerable improvement of symptoms for the spasmodic torticollis patients was observed with BTX-A treatment.The average dose of BTX-A was(120.37 ±25.26) U.Injection points were 30.00 ± 4.85.The Tsui scores before treatment were (13.08 ±4.16) scores,and 2 weeks after treatment were (4.21 ± 2.63) scores.The Tsui scores showed a significant reduction after BTX-A injections (P <0.01).After treatment complete remission rate was 25.0% (16/64),significant improvement rate was 60.9% (39/64),partial improvement rate was 12.5% (8/64),invalid rate was 1.6%(1/64) and efficient rate was 85.9% (55/64).Duration of effect was (16.86-4.57) weeks.Patients who received repeated injections also had good response,with symptoms improved and dosage of BTX-A reduced.No serious adverse events happened in treatments.Conclusion BTX-A therapy is simple and effective in the treatment of spasmodic torticollis and should be considered as the first-choice treatment for the condition.
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Objective To evaluate the clinical efficacy and safety of endovascular treatment for intracranial venous sinus thrombosis based on individual condition. Methods Twelve patients with intracranial venous sinus thrombosis were treated with endovascular management according to the severity and course of disease after they failed to respond to anticoagulant therapy. The clinical signs and symptoms,cerebrospinal fluid pressure and arteriovenous circulation time were observed and followed up (including MRV). Intravenous thrombolysis and mechanical thrombus maceration were carried out in all 12 patients,while intravenous thrombolysis, mechanical thrombus maceration in combination with intra-arterial thrombolysis were employed in 3. After the treatment, anticoagulant therapy was carried out for 6 months.The patients were followed up for 12 to 24 months. Results Of the twelve patients, clinical signs and symptoms included slight headache (2 cases), mild hemiplegia (1 case), ambiopia or blurred vision (3 cases). The cerebrospinal fluid pressure returned to under 26 cm H2O (1 cm H2O =0.098 kPa)following treatment from 28 to 38 cm H2O [ mean (32. 4 ±3.0) cm H2O] in preoperative measurement and the arteriovenous circulation time returned to below 10 s in all patients following treatment. Neither recurrence of thrombosis nor new symptoms of neurologic dysfunction was observed. No procedure-related intracranial or systemic hemorrhagic complications occurred both during and after the operation with the exception of a subcutaneous bleeding at the venopuncture site. Conclusion Endovascular treatment is effective and safe for patients with intracranial venous sinus thrombosis.
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@#Objective To investigate the protective effects of Naoxintong capsule on cerebral ischemia-reperfusion injury in rats.Methods 45 SD rats were randomly divided into 5 groups: sham operation group (n=5), ischemia-reperfusion group (n=10), small dose of Naoxintong capsule group (n=10), large dose of Naoxintong capsule group (n=10), CoQ-10 group (n=10). The the cerebral ischemia-reperfusion model was established by occluding bilateral carotid arteries of animal. The moisture content and activity of ATPase in brain tissue of model rat were observed respectively at ischemia 30 min plus reperfusion 30 min and ischemia 30 min plus reperfusion 60 min.Results The activities of Na+-K+-ATPase, Ca2+-ATPase and Mg2+-ATPase decreased (P<0.01), contents of water in brain tissues increased (P<0.01) in the ischemia-reperfusion group, compared with the sham operation group. The activities of Na+-K+-ATPase, Ca2+-ATPase and Mg2+-ATPase increased (P<0.01), contents of water in brain tissues decreased (P<0.01) in the Naoxintong capsule group, compared with the ischemia-reperfusion group.Conclusion Naoxintong capsule can protect the brain tissue from ischemia-reperfusion injury.