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In order to promote the innovative application of Sanjiao theory and Yingwei theory， this paper tries to apply the ''Sanjiao-Yingwei'' Qi transformation theory to the treatment of tumor diseases， integrating it with T cell exhaustion mechanism to elaborate on its scientific connotation and using network pharmacology and bioinformatics to elucidate the correlation between the anti-tumor mechanism of ''Sanjiao-Yingwei'' Qi transformation and T cell exhaustion. The ''Sanjiao-Yingwei'' Qi transformation function is closely related to the immunometabolic ability of the human body， and the ''Sanjiao-Yingwei'' Qi transformation system constitutes the immunometabolic exchange system within and outside the cellular environment. Cancer toxicity is generated by the fuzzy Sanjiao Qi， and the long-term fuzzy Sanjiao Qi is the primary factor leading to T cell exhaustion， which is related to the long-term activation of T cell receptors by the high tumor antigen load in the tumor microenvironment. Qi transformation malfunction of the Sanjiao produces phlegm and collects stasis， which contributes to T cell exhaustion and is correlated with nutrient deprivation， lipid accumulation， and high lactate levels in the immunosuppressed tumor microenvironment， as well as with the release of transforming growth factor-β and upregulated expression of programmed death receptor-1 by tumor-associated fibroblasts and platelets in the tumor microenvironment. Ying and Wei damage due to Sanjiao Qi transformation malfunction is similar to the abnormal manifestations such as progressive loss of exhausted T cell effector function and disturbance of cellular energy metabolism. Guizhi decoction， Shengming decoction， and Wendan decoction can correct T cell exhaustion and exert anti-tumor effects through multi-target and multi-pathways by regulating ''Sanjiao-Yingwei'' Qi transformation， and hypoxia inducible factor-1α （HIF-1α） may be one of the main pathways to correct T cell exhaustion. It was found that HIF-1α may be one of the important prognostic indicators in common tumors by bioinformatics. The use of the ''Sanjiao-Yingwei'' Qi transformation method may play an important part in improving the prognosis of tumor patients in clinical practice.
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Objective Resveratrol can improve nonalcoholic fatty liver disease , but its action mechanisms remain unclear . This study aimed to investigate the protective effect of resveratrol against the free fatty acid ( FFA)-induced apoptosis of human hepatic L02 cells and its possible mechanisms . Methods Human hepatic L02 cells were incubated with FFA and resveratrol for 24 hours.The prepared cells were divided into a blank control , an FFA ( 2 mmol/L) , and a resveratrol group ( 50 μmol/L resveratrol +2 mmol L/FFA).After treatment, we measured the triglyceride (TG), glutathi-one (GSH), and malonaldchyde (MDA) contents and caspase3 ac-tivity in the hepatocytes , determined the apoptosis of the cells by flow cytometry , and detected the protein expression of silent information regulator 1 (SIRT1) by Western blot as well as the mRNA expressions of catalase (CAT), Mn superoxide dismucase (MnSOD), Bcl-2, and Bax by qRT-PCR. Results The TG content and caspase 3 activity in the hepatocytes were significantly increased in the FFA ([3518.±64.2] μmol/L and [5.97 ±0.78] U/g) and the resveratrol group ([201.1 ±60.1] μmol/L and [3.60 ±0.73] U/g) as compared with those of the blank control ([40.2 ±7.4] μmol/L and [2.56 ±0.49] U/g) (both P<0.05), but the caspase3 ac-tivity was markedly decreased in the resveratrol group in comparison with that of the FFA group (P<0.05).Both early and late apop-tosis rates of the hepatocytes were remarkably higher in the FFA ([6.75 ±0.81]%and [8.52 ±0.54]%) and the resveratrol group ([4.94 ±0.44]%and [6.52 ±0.61]%) than those in the blank control ([3.38 ±0.33]% and [2.72 ±0.19]%) ( both P<0.05), with statistically significant differences between the former two groups (P<0.05).The resveratrol group showed significant differences in the GSH content ([100.2 ±8.8] nmol/g), the MDA level ([2.36 ±0.82] mg/g), and the relative expression of SIRT1 (0.84 ±0.04) from the FFA group ([73.8 ±13.1] nmol/g, [3.77 ±0.92] mg/g, and 0.61 ±0.07) and the control ([113.7 ±13.8] nmol/g, [1.85 ±0.41] mg/g, and 0.90 ±0.02) (all P<0.05).The resveratrol group also exhibited statistically significant differences in the relative expressions of the MnSOD , CAT, and Bax genes from the FFA and control groups (P<0.05). Conclusion Resveratrol attenuates FFA-induced apoptosis of human hepatic L 02 cells by activating SIRT1 and reducing the oxidative stress of hepatocytes .
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The retrospective analysis of data of snail status in Baofeng harbor,Yingcheng City from 1975 to 2009 suggests that the snail surveillance in schistosomiasis transmission-interrupted areas is a long term work,which should be strengthened continuously.
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[Objective] To observe the effect of Zhuyun Decoction on immune sterility rats.[Method] Set up groups of normal saline,model,Zhuyun Decoction and prednisone,make models of every group by injecting subcutaneously suspension of sperm and Fu's adjuvant,etc.into female SD rats along the groin,NS into normal saline group.After 4 weeks,the groups of normal saline and model take NS,Zhuyun Decoction for Zhuyun Decoction group,prednisone for prednisone group.After 2 weeks,they are closed together with male ones.Before the first immune and 1 week after strengthened immune,take blood and measure AsAb with ELISA method,measure the immune integral of IgG and IgA in uterus tunica intima with SABC method;meanwhile,compare the pregnant rats and average nidation points in all groups.[Result] In the Zhuyun Decoction group,the pregnant rats and nidation points are much more that other groups;Zhuyun Decoction can reduce IgG and IgA integral in uterus tunica intima.[Conslusion] Zhuyun Decoction has definite effect on immune sterility rats,whose mechanism may be related with regulation of IgG and IgA integral in uterus tunica intima.
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[Objective]To explore the effect of combined therapy of Chinese and western medicine on Bell paralysis.[Methods] Randomly divide 62 cases of Bell paralysis into 2 groups who were treated respectively with combined therapy of Chinese and western medicine and western medicine.[Results] For effective rate,the treatment group(91.1%) was markedly higher than control group(67.8%),there was obvious difference between the 2 groups.[Conclusions] Combined therapy of Chinese and western medicine has marked therapeutic effect on Bell paralysis.
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To evaluate the effect of Vitamin E on mitoc bond rial function of cold exposed rats male weaning rats were divided randomly into three groups. The group A was supplemented with 30 mg ?-tocopherol and 15?g sodium selenite per 100gm basal diet. The group B and C were fed basal diet on- ly. The group A and B were kept at the cold room (-2?1℃) and the group C kept at room temperature. Liver mitochondria were isolated for measurement of its respiratory function. Succinate was utilized as substrates of oxidative phosphorylation. Respiration rates of state 3 oxygen uptake and therefore ATP synthesis were found to increase with Vitamin E supplemented diet. Significantly less decline of ADP/O ratios and RCR was observed for group A than that of group B. These results suggest that Vitamin E has the effect of maintaining the physiological intactness of mitochondria of the cold exposed rats, and therefore may enhance the metabolic conservation of energy with the consequence of increasing the ability of cold tolerance of the cold exposed animals.