ABSTRACT
Objective:To study the feasibility of 3-D reconstruction model in the observation of tetrachlorodibenzo-p-dioxin(TCDD) effected palatal organ development of fetal mouse.Methods:Kunming mice treated 40 ug/kg TCDD by lavage on day 12.5 of pregnancy were used as in the experimental group,isodose corn oil treated in the control group.On day 13.5,14.5 and 15.5 of pregnancy heads of the fetal mice were taken out and fixed.Conventional paraffin serial sections of palatal organ were preparated and dyed by hematoxylin-eosin,images of the palatal organs were collected and photoshop treated,3-D reconstruction of the palatal organ was performed by 3D-DOCTOR software.Results:3-D reconstruction images showed that palatal organs moved from on both sides above the tongue and gradually closed and merged in the control group.In the experimental group,the palatal organs moved from on both sides above the tongue was later than control group,gradually closed,but not merged,formed cleft palate.Conclusion:3D-DOCTOR software reconstruction can be used for the study of the development process effected by TCDD in the pregnant mouse.
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Objective To investigate the plasma total tau(T-tau)and phosphorylated protein tau(P-tau)protein in the diagnosis of Alzheimer's disease(AD).Methods 22 352 medical patients aged over 60 years in Beichen Area Community Service Center were involved in this survey.Random sampling principle was used for screening.Mini-mental state examination(MMSE)and activities of daily living assessment form(ADL)were conducted for the cognitive function and ability of daily score firstly.When the score below the standard,the Hamilton depression scale and Hachinski ischemic scale were re-used for diagnostic score.Related laboratory tests were conducted to exclude other central nervous system and other systems and material causes of dementia patients.Ultimately,105 cases of AD,diagnosed by the neurologist,and 42 cases of non central nervous system disease,non dementia non nervous system disease patients as the normal control group were involved.T-tau and P-tau levels in the two groups were determined by ELISA.Results the T-tau concentration in AD group(15.93+6.59)ng/L was higher than control group(14.10±6.32)ng/L,no significant difference was found (P ＞ 0.05).However,compared with control group (0.69 ± 0.24) ng/L,P-tau protein in AD group (1.26 ± 0.75)ng/L increased significantly(P＜0.05).Conclusion Plasma levels of phosphorylated tau protein might have diagnostic value for AD patients.
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Objective To evaluate clinical efficiency of Tongxinluo capsule in the treatment of stable angina pectoris.Methods Patients(n=98) were randomLy divided into two groups.The treatment group of 50 patients received Tongxinluo capsule 1.14g,po,tid for 4 WK.The control group of 48 patients received Isosrbide dinitrate 10 mg,po,tid for 4 WK.Results The clinical and electrocadiogram(ECG) effective rates of the treatment group were 70.0% and 48.0%respectively,which were significantly higher than 45.8%and 22.9%of the control group,P