ABSTRACT
Objective: To study the effects of zinc on transport function of erythrocyte membrane. Methods: This study was conducted both in vivo and in vitro. In vivo, weanling rats were divided into three groups and fed with different zinc diets (2.2, 28 and 128 mg Zn/kg diet) for four weeks, the transport function of Na +/K + pump, COTS-1, COTS-2, Gardos and RF channels were determined. In vitro, different concentration of zinc (0,5,10,50,100 and 500 ?mol Zn 2+ /L) were added into fresh human blood and the activities of the five transport channels were detected. Results: Proper zinc could keep the highest activities of Na +/K + pump, COTS-2 and Gardos channel. Too low or too high zinc decreased the transport function of these three channels and the activities of COTS-1 and RF channel were increased with the increase of zinc concentration, indicating the competitive function of these two channels. Conclusion: Zinc plays an important role in maintaining the transport function of erythrocyte membrane.
ABSTRACT
Objective: To investigate the specific effects of resistant starch (RS) on zinc status in rats. Methods: (1)Zinc metabolism in normal rats: Three groups of Wistar rats were fed with basal diet (control), diet with 13% or 26% RS respectively, for 18 days. Urine, fecal and blood samples were collected for zinc measurement. Zinc apparent absorption was calculated.(2)Zinc status in rats fed with high sucrose (50%) diet: Three groups of Wistar rats were fed with basal diet, high sucrose digestible starch diet (S DS) or high sucrose resistant starch diet (S RS, containing 14% RS) respectively for 12 w. Samples of urine, blood, liver, pancreas and kidney were collected for measurement of zinc content. Results: (1)Zinc apparent absorption in normal rats was: control group 56.59%, 13%RS group 50.11%, and 26%RS group 54.40%. (2)High sucrose diet led to increased postprandial glucose and HbA 1C and depressed fast insulin level in S DS group rats, with decreased plasma and pancreas zinc level (compared with control group, P
ABSTRACT
Objective: To observe the effect of yoghurt supplementation on the growth rate of preschool children in Beijing suburb. Methods: 402 healthy children (217 males, 185 females), aged 3-5 years old, were selected as subjects with height and/or weight less than WHO standard from 7 kindergartens in Beijing suburb. The children were divided randomly into control group (CG 201) and yoghurt supplemented group (YG 201). Children in YG were supplemented with yoghurt (125g) for 5d per week for 9 mo, and children in CG not supplemented. Height, weight and upper-arm circumference were measured every month. Blood biochemical parameters (Hb,TP) were detected every 3 mo. Results: Calcium, zinc and VB2 were consumed more in YG than CG. Hb in YG was also higher than CG . The height incease and weight gain of YG was significantly higher than CG during 3, 6 and 9mo. Conclusion: Yoghurt supplementation for 9 months can increase calcium, zinc and VB2 intake, and improve preschool childrens nutritional status, therefore increase their height increase and weight gain.