Primary pleural diffuse large B-cell lymphoma accompanied with immune thrombocytopenia: report of one case and review of literature / 白血病·淋巴瘤

Objective@#To explore the clinical features, laboratory examinations and diagnosis progress of primary pleural diffuse large B-cell lymphoma (DLBCL) and analyze the relationship between immune thrombocytopenia (ITP) and lymphoma, and to improve the diagnostic level of the rare disease.@*Methods@#The clinical features, diagnosis and treatment of one primary pleural DLBCL patient with ITP in Ruijin Hospital of Shanghai Jiao Tong University School of Medicine were observed. The related literatures were also reviewed.@*Results@#The patient was admitted to the hospital due to repeated chest tightness and shortness of breath for 6 months. Ultrasonic examination, chest CT scan, PET-CT and pleural effusion puncture were performed. The main clinical feature was only pleural effusion without hepatosplenomegaly, enlargement of lymph nodes and any other lesions. Pathological study of pleural effusion confirmed the diagnosis of DLBCL. The patient was suffered from ITP for 30 years. After confirmed diagnosis, 6 courses of R-CHOP regimen had been performed. Mid-term assessment showed the complete absorption of pleural effusion and achieved complete remission.@*Conclusions@#Primary pleural DLBCL is a rare disease. It is easy to misdiagnose due to the lack of specificity in clinical and imaging manifestations. The final diagnosis ultimately depends on histopathology. ITP can occur prior to lymphoma or onset of lymphoma, and a few patients can present with ITP in the course of lymphoma.

Effect of dexmedetomidine on proliferation, migration and invasion of human gastric cancer SGC-7901 cells / 临床麻醉学杂志

Objective To observe different behavior of proliferation,migration and invasion of SGC-7901 cells when exposured to dexrnedetomidine of different concentrations.Methods Human gastric cancer cells SGC-7901 were inoculated on culture plate for 24 h,then were randomly divided into 5 groups:control group (group C),dexmedetomidine 312.5μg/ml group (group D1),dexme detomidine 625μg/ml group (group D2),dexmedetomidine 1 250 μg/ml group (group D3),dexmedetomidine 2 500 μg/ml group (group D4).Each group was medicated and incubated for 48 h,then the cell proliferation,migration and invasion immediately were detected by CCK-8 and Transwell.Results SGC-7901 cell viability of groups D1,D2,D3 和 D4 had no significant difference compared with that of group C.The invasion ability and migration ability of SGC-7901 cells in groups D1,D2,D3 and D4 were significantly higher than those in group C (P ＜ 0.05 or P ＜ 0.01).Conclusion Dexmedetomidine can promote migration and invasion of SGC-7901 cells.

A Fiber-Optic Sensor Based on Plasmon Coupling Effects inGold Nanoparticles Core-Satellites Nanostructures for Detection of Mercury Ions / 分析化学

Based on the plasmon coupling effect in gold nanoparticles core-satellite nanostructures linked by thymine(T)-rich DNA hybridization and the specific Hg2+-mediated T-Hg2+-T base pair, a novel localized surface plasmon resonance (LSPR) optical fiber sensor was proposed and developed for Hg2+ detection in water.The Hg2+-induced conformational change in T-rich DNA sequence inhibited the DNA hybridization reaction, weakened the plasmon coupling effect and leaded to the change of LSPR resonance wavelength.The concentration of Hg2+ was quantitatively determined by the resonance wavelength redshift.The linear range of Hg2+ detection was about 5-150 nmol/L with LOD about 3.4 nmol/L.The specificity of the sensor was proved great by evaluating the response to other heavy metal ions such as Zn2+, Mg2+, Pb2+ and so on.This sensor was applied in environmental water detection by standard addition method,with the RSD less than 4.8% and recoveries of 94.2%-105.4%.

Effect of continuous incision infusion different concentration of ropivacaine for postoperative analgesia after radical mastectomy / 临床麻醉学杂志

Objective To observe the effect of continuous incision infusion different concentra-tion of ropivacaine for postoperative analgesia after radical mastectomy.Methods One hundred pa-tients under radical mastectomy,aged 40-70 years,ASA Ⅰ or Ⅱ,were randomly divided into four groups (n =25 each):0.2% (group R1),0.3% (group R2),0.4% (group R3)ropivacaine incision continued infiltration group and patient-controlled intravenous analgesia (group PCIA)as control group.VAS pain scores,sedation Ramsay score and side effects were recorded at each time point in rest and turning over 90°,2 h (T1 ),4 h (T2 ),8 h (T3 ),12 h (T4 ),24 h (T5 ),48 h (T6 )after the operation.Results VAS scores in group R1 at T1-T6 in rest and turn over 90°were significantly high-er than that of group PCIA (P <0.05).There were no significant differences among the group PCIA, group R2 and group R3.Sedation score in PCIA group was significantly higher than that in the other three groups (P <0.05),and the adverse reactions,such as nausea and vomiting,in group PCIA (2 cases)were more serious than that in the other groups (0 cases ).There were no significant differences among the other groups.Conclusion Ropivacaine plays an effective role in infiltration an-algesia when its concentration reaches 0.3% subcutaneous after radical mastectomy.

The effect of ropivacaine on proliferation and cell cycle of human breast cancer MCF-7 cells / 临床麻醉学杂志

Objective To observe the different behavior of proliferation and cell cycle of MCF-7 cells when exposured to ropivacaine of different concentrations and further explore its underlying mechanism.Methods Human breast cancer cells MCF-7 were inoculated into culture medium for 24 h,then were randomly divided into four groups:Control group(group C),Ropivacaine 100 μg/ml group(group R1 ),Ropivacaine 200 μg/ml group(group R2),Ropivacaine 400 μg/ml group(group R3).We medicated each group and incubated for 48 h,then detected the cell proliferation and cell cy-cle immediately.The level of protein TCF-4 and beta-catein of groups R3 and C were measured at the same time.Results MCF-7 cell viability of groups R2 and R3 was significantly lowed (P <0.05 ), MCF-7 cell viability of group R1 had no significant difference when compared to group C.G0/G1 phase cells of groups R1,R2 and R3 were significantly less than those of group C,S phase cells of groups R1,R2 and R3 were significantly more than group C,G2/M phase cells of groups R1,R2 and R3 were significantly more than group C (P <0.05).The expression level of TCF-4 and beta-catenin in group R3 was significantly lower than that in group C (P <0.05).Conclusion Ropivacaine inhibits the proliferation of breast cancer cells MCF-7 by down-regulating TCF-4 and beta-cateni.

A Micro Electrochemical Sensor for Nitrate Determination Based on Square_wave Pulsating Current Deposition Process / 分析化学

Based on microfabrication technology and electrochemical modification method, a micro electrochemical sensor for nitrate ( NO-3 ) determination was developed. A micro sensor chip with working electrode and counter electrode was used as the signal convertor of the sensor. The area of the micro working_electrode was only 1 mm2 . As an electrocatalysis sensitive material, copper was electrodeposited onto the working electrode by square_wave pulse current electrodeposition method. The morphologies and components of freshly deposited materials were examined by scanning electron microscopy ( SEM ) and X_ray diffraction ( XRD) to explore key factors that affected the electrocatalytic ability of the deposited copper layer for reducing nitrate ions. The experimental results revealed that under the optimal conditions, the deposited copper layer was macroporous and had a larger effective surface area that could serve as a more effective electrocatalyst in facilitating nitrate reduction. Electrochemical response of the macroporous copper layer was characterized by linear sweep voltammetry in acidic supporting electrolytes ( pH=2 ) . The electroanalytical results showed that the modified microsensor had marked sensitivity for standard nitrate samples within the concentration range from 12. 5 to 3000 μmol/L (in the range of 12. 5-200 μmol/L yielded straight line:y1=-0. 1422x-10. 326, R12=0. 9976, while in the range of 200-3000 μmol/L yielded straight line: y2=-0. 0984x-22. 144, R22=0. 9927) with a detection limit of 2 μmol/L (S/N=3). The developed electrochemical microsensor was also employed for nitrate determination in water samples collected from lakes and rivers near the city of Beijing. The results were in good agreement with the data given by qualified water quality detection institute, with the deviations from 3 . 9% to 15 . 4%.

Mechanisms regulating p21 gene expression by retinoic acid-induced gene G protein / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the mechanisms by which retinoic acid-induced gene G (RIG-G) protein regulates p21 gene expression.</p><p><b>METHODS</b>Western blot was used to detect the effects of RIG-G protein overexpression on p21 protein expression level in leukemia cell line NB4 cells and the phosphorylation of both c-Jun and JNK in U937 cells. The c-Jun expression plasmid and p21 gene promoter-containing reporter plasmid were co-transfected into 293T cells, to explore the regulatory effect of c-Jun protein on p21 gene expression by luciferase reporter assay.</p><p><b>RESULTS</b>Western blot showed that the overexpression of RIG-G protein significantly upregulated p21 protein level in the NB4 cells, and the level of p21 protein largely increased along with the induction of endogenous RIG-G protein during the differentiation of NB4 cells treated by all-trans retinoic acid (ATRA). Moreover, the phosphorylation of both c-Jun and JNK decreased in RIG-G-overexpressing U937 cells while total c-Jun and JNK proteins remained unchanged. After using the JNK inhibitor SP600125 to block JNK phosphorylation, the level of c-Jun phosphorylation was still dramatically reduced in the RIG-G-overexpressing U937T-RIG-G cells, compared with the control U937T-pTRE cells. These results indicated that the inhibitory effect of Rig-G protein on c-Jun phosphorylation could not only be through the JNK pathway, but also via some JNK-independent pathways. Luciferase reporter assay showed that when 0.1, 0.5, 1.0 and 2.0 µg c-Jun-expressing plasmids were respectively transfected into 293T cells, compared with the empty vector-transfected group, the relative luciferase activities were (83.0 ± 1.7)%, (73.7 ± 0.7)%, (68.9 ± 0.9)% and (64.1 ± 0.9)%, indicating that the transcriptional activity of p21 gene could be inhibited by c-Jun protein.</p><p><b>CONCLUSIONS</b>RIG-G protein may suppress the phosphorylation of c-Jun protein through different signal pathways, thereby increasing the expression of p21 gene, arresting the cell cycle and inhibiting the cell growth in U937 cells.</p>

The effects of morphine combined with cisplatin on invasionon and migration of human lung adenocarci-noma cell A549 / 临床麻醉学杂志

Objective To investigate the effect of morphine combined with cisplatin on inva-sionon and migration of human lung adenocarcinoma cell A549.Methods Human lung adenocarcino-ma A549 cells were inoculated on cultured for 24 h,then were randomly divided into 5 groups:control group (group CON),cisplatin group (group CIS),morphine 0.3 μg/ml+ cisplatin group (group MT1),morphine 3 μg/ml+ cisplatin group (group MT2),morphine 30 μg/ml+ cisplatin group (group MT3).Cisplatin concentration was 4μg/ml.Each group was medicated immediately after 48 h incubation,invasion detection cells by Transwell assay,cell scratch assay cell migration ability, Western-blot detection of matrix metalloproteinase-2 (MMP-2),the expression of MMP-9,Ezrin protein and Fascin protein.Results Compared with group CON,group CIS and morphine combined with cisplatin group reduced tumor cell invasion and migration ability,group MT3 and CIS down-reg-ulated MMP-2,MMP-9,Ezrin and Fascin expression (P<0.05).Compared with group CIS,com-bined with cisplatin group enhanced tumor cell invasion and migration ability group MT3,up-regula-ted MMP-2,MMP-9,Ezrin and Fascin expression (P<0.05).Conclusion Morphine may dose de-pendently reduce cisplatin on invasion and migration of human lung adenocarcinoma cell line A549, and up-regulation of MMP-2,MMP-9,Ezrin,Fascin expression is one of its possible mechanisms.