ABSTRACT
Objectives:To evaluate the effects of PEDF eyedrops on the corneal allograft rejection by topical installition in the rats model of penetrating keratoplasty,and to investigate its possible mechansism and its correlation factor.Methods:Penetrating keratoplasty(PKP)was proformed orthotopically in wistar rats and SD rat recipients.60 SD rats were randomly assigned to two groups;experimental group and experimental control group after PKP.30 SD rats underwent auto penetrating keratoplasty(blank control group).The experimental group was treated with PEDF eyedrops,while the blank control group and experimental control group were treated with escipient eyedrops.All animals'surrival time was recored and compared by the biomicroscope examination.6 animals in three groups were killed respectively at the 1st week,2nd week,3rd week,4th week and 5th week postoperatively.Corneal ultrastuctural morphology was observed by TEM.PEDF and VEGF expression was examined by immunohistochemistry.Results:Expression level of VEGF of experimental control group was higher than that of experimental group(P
ABSTRACT
Objective To explore the temporal expressions of TIMP-1 and their relationship with injury time during electric injuris of hearts of rats.Methods Immunohistorichemical and image-analysis techniques were employed in 220V-electric injury of hearts during different time.ResultsWeak expression of TIMP-1 are deteced at 0.5h after electric injury,which increased subsequently,and peaked at 24~72h post-injury.Then decreased to pre-injury level at 12d.Conclusion The time-dependent expression of TIMP-1 during electric injury may be used for the estimation of injury time.