Analysis of sub-health symptom and health risk behavior factors among left behind middle school students in Chongqing three-gorge area / 重庆医学

Objective To study the sub-health status and health-risky behaviors of left behind middle school students in Chongqing Three-gorge area and provide the reference for targeted interventions .Methods 1 528 cases of left behind middle school students were collected from three middle school by multistage cluster random sampling in a reservoir of Chongqing ,surveyed with questionnaire about health-risky behaviors and Multidimensional Sub-health Questionnaire of Adolescents(MSQA) .Results The rates of psychosomatic sub-health symptoms were 45 .0% ,body sub-health symptoms detection rate was 23 .3% ,psychological sub-health symptoms detection rate was 40 .1% .Body energy insufficiency ,the physiological function ,decreased resistance detection rate were 10 .2% ,15 .1% and 12 .0% ,respectively .The emotional problems ,behavioral problems ,social adaptation difficulties detection rate were 25 .9% ,16 .8% ,34 .3% ,respectively .The symptoms of sub-health detection rate of boys was higher than that of girls (P>0 .05) ,the junior grade One was higher than the senior grade One(P0 .05) ,the boarding students was significantly higher than the day students(P<0 .05) .Logistic regression analysis showed that the risk factors had unhealthy diet behavior ,deliberately harm behavior ,suicide behavior ,unintentional injury behavior and Internet addiction .Conclusion The sub-health problem of left behind middle school student in Chongqing three-gorge area is in a general level ,the relevant departments should take some targeted interventions to improve students'health level .

Preparation and quality evaluation of ginkgolide B-loaded self microemulsifying drug delivery system / 中国中药杂志

<p><b>OBJECTIVE</b>To prepare ginkgolide B-loaded self microemulsifying drug delivery system (SMEDDS) and evaluate its quality.</p><p><b>METHOD</b>The solubility of ginkgolide B in different oil, surfactant and co-surfactant were measured by HPLC-ESI-MS. The GB-SMEDDS formulation was optimized by the self emulsifying efficiency of various combinations of oil and mix-surfactant evaluated by using pseudo-temary phase diagram. The preliminary stability of GB-SEMEDDS was evaluated by the variety of loading rate of GB and dispersed medium. The morphology, the particle size and the formulation stability were evaluated after diluting by 0.1 mol x L(-1) HCl.</p><p><b>RESULT</b>The blank self microemulsified system was composed of ethyl oleate-( cremophor EL-lecithin-ethanol, 4: 1:2) (40: 60), the loading dosage was 2.5%. Little influence of GB and emulsified medium was observed on the stability of GB-SEMDDS. After diluted with 0.1 mol x L(-1) HCl, the morphology of the microemulsion was homogeneous small spherical drops observed under electro-microscope. The particle size was (41.6 +/- 1.11) nm, the self microemulsifing time was around 2 min. The formulation was stable within 8 h, without significant changes in particle size and separation of drugs.</p><p><b>CONCLUSION</b>GB-SMEDDS is easy to prepare and its quality is stable. The solubility of GB was significantly improved by SMEDDS.</p>

Characterizations of human retinal microvascular endothelial cells at various ages / 中国病理生理杂志

AIM: To investigate the properties of human retinal microvascular endothelial cells (RMECs) at two different age groups. METHODS: Human RMECs with high affinity were isolated from donors at two age groups: 30 d (group A) after birth and 40 - 60 years of age (group B). The RMECs were characterized for expression and localization of endothelial cell markers by immunofluorescence staining of CD31, yon Willebrand factor(vWF) and uptake of acetylated low - density lipoprotein. The ability of tube formation was assessed on Matrigel, and vWF distribution in- cells was ob-served by confocal immunofluorescence microscope and Western blotting analysis, respectively. RESULTS: High purity RMECs can be obtained readily from each group with modified methods. At 6 hours, cells from both groups formed tube structures successfully, but there was a significantly higher incidence of branching in RMECs of infant group (group A) by 27.2%±2.2% compared with adult group (group B) at 12 h (P<0.05). Group A maintained intact structure even at 30h, but group B partially lost the basic tube structure. In addition, vWF was translocated from cytoplasm to nucleus with aging. CONCLUSION: Human RMECs at different ages have specific properties. Cell properties related with age of the donors should be considered in in vitro studies.

Preparation of Rapid-disintegrated Lozenge / 中国药房

OBJECTIVE:To prepare a rapid-disintegrated lozenge METHODS:The formula and technology were decided by taking the disintegrating time and dissolubility as standard RESULTS:The lozenge could completely disintegrate in 20s and the accumnlated dissolubility of the model drug was 99% after 5 minutes in phosphate buffer(pH=6 80) CONCLUSION:Using now available equipment,suitable excipients and workmanship can prepare a rapid-disintegrated lozenge