ABSTRACT
Objective:To explore the value of a holistic communication program for inpatients developed based on the medical humanistic GLTC communication model, designed to improve patient satisfaction and medical quality, and effectively enhance doctors′ communication and clinical work capabilities.Methods:A GLTC Doctor-Inpatient Communication Program and an Inpatient Satisfaction Questionnaire for Doctor-patient Communication were customized.From August 2018 to February 2019, the communication program was used to train doctors from different departments of five tertiary public hospitals in Jiangsu province. The questionnaire was used to survey both doctors and their inpatients. 250 questionnaires were distributed each time, to compare the doctor-patient satisfaction before and after the training and influencing factors. Data so acquired were subject to descriptive analysis, t-tests and multiple linear regression analysis. Results:Since application of the program, patient satisfaction scoring was higher than that before in general( P<0.05), while the patient satisfaction for medical expenses and disease explanation communication related programs was low. The difference of both doctors and patients satisfaction scoring for doctor-patient communication was statistically significant( P<0.05). Oncologists presented a higher evaluation on the applicability of the program than those from other departments( P<0.05). Conclusions:Application of the GLTC doctor-patient communication program can effectively improve the satisfaction of inpatients. It is recommended that this communication program be integrated into the entire medical treatment of inpatients, and the implementation plan should be differentiated according to the differences of inpatients. It is necessary to strengthen the communication of medical expenses and explain medical knowledge to inpatients, while doctors′ mindset of consultation should be adapted, with the program constantly improved for higher clinical applicability.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the genetic diversity and structure of Dendrobium huoshanense, a (CT)n enriched microsatellite library was constructed using a magnetic beads enrichment procedure.</p><p><b>METHOD</b>The 3'-biotinylated oligonucleotide probe was used to hybridize with the digested D. huoshanense genomic DNA fragments whose both ends were ligated with adaptors. The hybridized complex was then combined with the streptavidin-coated magnetic beads. The captured microsatellite fragments were eluted, collected and cloned into pMD19-T vector. The recombinant plasmids were transformed into Escherichia coli DH5alpha competent cells. The clones that yielded two or more bands contained microsatellite fractions. Positive clones were screened and sequenced. Thirty pairs of primers were designed and synthesized. Polymorphism at each locus was determined using 24 individuals from a natural population from Huoshan county town in Anhui province.</p><p><b>RESULT</b>Twelve polymorphic microsatellite loci from the microsatellite-enriched genomic library were newly developed across 24 D. huoshanense individuals. In total, 65 alleles were identified, and the number of alleles per locus ranged from 2 to 8. The mean observed and expected heterozygosities were 0.500 and 0.638, respectively. Two loci significantly deviated from Hardy-Weinberg equilibrium (P<0.05), which could be due to the presence of null alleles. Furthermore, three of twelve loci showed significant linkage disequilibrium (P<0.05).</p><p><b>CONCLUSION</b>These results suggest that the identified polymorphic microsatellite markers will be useful in population genetic studies of D. huoshanense.</p>