ABSTRACT
Objective To investigate the expression of peroxisome proliferator-activated receptor γ (PPARγ) and uncoupling protein-1 (UCP-1) after sleeve gastrectomy in Zucker rats and to discuss the weight loss mechanisms.Metbods 30 male Zucker rats aged 10 weeks were randomly divided into 3 groups:the operation group (10 rats),the sham operation group(10 rats) and the diet-pairing group (10 rats).The rats were decapitated to retrieve the retroperitoneal adipose.mRNA and protein expressions of PPARγ and UCP-1 were detected by RT-PCR and Western blot.Results As for the operation group,the weight decreased significantly after the operation compared to the other two groups((250±5.8) g,(370±10.0) g,(310±9.6) g,respectively,P＜0.05).The expressions of PPARγ and UCP-1 gene of mRNA and protein were all significantly higher in the operation group (P＜0.05).Conclusions SG can up-regulate the expressions of thermogenic gene PPARγand UCP-1 in adipose in Zucker rats,browning the white adipose tissue,which was one of the important mechanisms of weight loss.
ABSTRACT
Objective To investigate the effect of sleeve gastrectomy (SG) on the distal ileum apical sodium-dependent bile acid transporter (ASBT) and ileum bile acid binding protein (IBABP)in obese type 2 diabetic rats.Methods Thirty 8-week-old male Sprague-Dawley rats were divided into groups O,F and N according to the random number table method.Among them,groups O and F were given high-sugar and high-fat diets,and group N were fed with normal diets before and after surgery.After 1,2 and 4 weeks,the body weight,blood glucose and plasma total bile acid levels were measured.The experimental animals were sacrificed 4 weeksafter operation and the terminal ileum tissue was harvested.Two types of ASBT and IABAP were detected by Western blot and immunohistochemistry.Bile acid transporters were tested.Results Blood glucose in group O was significantly lower than that in group F at 4 weeks postoperatively,(5.8±1.77) and (19.55±2.16) mmol/L,respectively (P＜0.05).Body weight in group O was significantly lower than that in group F at 4 weeks postoperatively,(182±10.66) g and (362±23.54) g,respectively (P＜0.05).Plasma total bile acid levels in group O were significantly higher than those in group F and N at 4 weeks after operation (52±18.48),(6.39±1.18),(5.89±1.21)μmol/L,respectively(P＜0.05).At the end of the 4th postoperative period,the expression of ASBT and IBABP in the distal ileum of group O increased,but there was no significant change in group F and group N.Conclusions The effect of SG on the hypoglycemic and weight-loss of obese T2DM rats is definite.The level of bile acid in plasma increases significantly after SG,and the expression of bile acid transporter in the terminal ileum of rats is one of the mechanisms.
ABSTRACT
Objective To investigate the expression of hormone sensitive lipase (HSL) and carnitine acyl transferase-1 (CAT-1) in Zucker rats after sleeve gastrectomy and to discuss the weight loss mechanism.Methods 30 male Zucker rats aging 10 weeks were randomly divided into 3 groups:the operation group (10 rats),the sham operation group (10 rats) and the diet-pairing group (10 rats).The rats were decapitated to retrieve the retroperitoneal adipose,mRNA and protein expression of HSL and CAT-1 gene of were detected by RTPCR and Western blot.Results As for the operation group,the weight decreased significantly after the operation comparing to the other two groups ((250±5.8) g,(370±10.0) g,(310±9.6) g,P＜0.05).mRNA and protein expressions of HSL and CAT-1 gene were all significantly higher in the operation group (P＜0.05).Conclusion SG can up-regulate the expressions of lipolysis gene HSL and CAT-1 in adipose in Zucker rats,promoting fat hydrolysis and increasing the energy expenditure,which is one of the important mechanisms of weight loss.