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【Objective】 To investigate the environmental pollution of blood collection and supply institutions by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and evaluate its application value. 【Methods】 Colonies of air from blood donation sites, skin puncture sites of blood donors, platelet storage boxes, platelet collection equipment, object surfaces of related experimental consumables and cuff surfaces of staff after disinfection were collected, and typical colonies after cultivation were selected for microbial identification by microbial mass spectrometry and then compared with bacteria results detected in blood components from May 2017 to May 2021. 【Results】 Aseptic growth, the number of colonies ≤4.0 CFU/ dish, and the number of colonies > 4.0 CFU/dish accounted for 21.20%, 62.20% and 16.60%, respectively. The qualified rate from high to low was platelet storage box, bacteria settling in the air of blood donation room after disinfection, platelet collection equipment, skin puncture site of blood donors after disinfection, the surface of platelet consumables and the surface of medical staff's overalls. After disinfection, the blood donors' skin puncture sites were compared with other collection sites, and the t values were 2.0371, 1.508, 2.109, 1.961 and 1.778, respectively, with no significant difference (P>0.05). Thirty cases of bacterial contamination of blood components were detected from May 2017 to May 2021, among which the detection rate of apheresis platelets was the highest, and the t values were 1.731 and 2.272, relative to the contamination frequency of erythrocytes and plasma bacteria (P>0.05), while the t value was 2.875, relative to concentrated platelets, with significant difference (P<0.05). 【Conclusion】 Bacterial contamination of blood components mostly come from air bacteria settling, blood donors' arms and skin after disinfection, and surfaces of related equipment and materials. Therefore, it is of clinical significance to conduct strict disinfection of working sites, establish disinfection monitoring methods and formulate disinfection hygiene standards in blood stations.
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Since its founding 100 years ago, the Communist Party of China (CPC) has attached great importance to the occupational health of workers, and closely linked the safeguard of occupational safety and health of workers with the missions of leading the people to stand up, get rich, and become strong. Based on this, the achievements of occupational health work made under the leadership of the CPC since the new democratic revolution, socialist revolution and construction, the new period of reform and opening up and socialist modernization, and socialism with Chinese characteristics for a new era were summarized, so as to provide reference for understanding the legacy of China's occupational health undertaking, and innovating to create the future.
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Objective To investigate the experience and effect of emergency cosmetic surgery for maxillofacial trauma.Methods From April 2011 to March 2017,256 patients with maxillofacial trauma in emergency cosmetic surgery in the People's Hospital of Lezhi County were selected in the study.The causes,locations,treatment methods and clinical effects were analyzed.Results In 256 cases,125 cases of traffic accident injury,68 cases of agricultural and industrial injury,48 cases of accidental injury,13 cases of asfights,2 cases of animal bites;of which 121 cases of lower face,73 cases of midface,39 cases of midface and lower face together,23 cases of upper face;98 cases of maxillofacial region fracture;105 patients had different degree of soft tissue defect.All patients the wound healed in primary healing,after stitches the wound healing in good contraposition,surface leveling,scar slender,facial features did not change significantly,no complications were observed.After 3-6 months of follow-up,the scar was not obvious and the appearance was satisfactory.Conclusion The clinical effect of emergency cosmetic surgery for maxillofacial trauma is satisfactory.Emergency cosmetic surgery can be used as an emergency treatment for maxillofacial trauma in primary hospital.
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Objective To analyze gene expression profiles of biopsy specimens from breast cancer patients who were treated with neoadjuvant chemotherapy(NAC) after biopsies, and to identify the genes which are closely associated with the efficacy of neoadjuvant chemotherapy with T/FAC [docetaxel(Taxotere), 5-fluorouracil, doxorubicin and cyclophosphamide] or T/FEC (Taxotere, 5-fluorouracil, epirubicin and cyclophosphamide) regimen.Methods We retrieved and collected gene expression profiles from publicly available databases.Four datasets, a total of 844 samples, were finally retained because all the patients had received a uniform neoadjuvant chemotherapy regimen.Response to neoadjuvant chemotherapy was categorized as a pathological complete response (pCR) or residual invasive cancer (RD).The differentially expressed genes (adjusted P-value<0.05) and therapeutic efficacy were analyzed and explored.Results After differential analysis, genes whose expressions were higher or lower in pCR group than in RD group were identified in each of the four datasets, respectively.There were 34 and 42 genes which were simultaneously more highly expressed or more lowly expressed in pCR group than in RD group in the four datasets.The unsupervised clustering, based on the 76 intersection genes, showed that the pCR specimens tended to form one cluster and the RD tended to form the other.Conclusion The seventy-six differentially expressed genes are associated with the efficacy of neoadjuvant chemotherapy and are likely to be novel predictive biomarkers for the efficacy of neoadjuvant chemotherapy.
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<p><b>OBJECTIVE</b>To evaluate the alveolar bone thickness and root length changes of anterior teeth with cone-beam computed tomography (CBCT).</p><p><b>METHODS</b>CBCT scans were taken for 12 skeletal Class III patients who accepted the improved corticotomy (IC) procedures during pre-surgical orthodontics. The CBCT data in T1 (the maxillary dental arch was aligned and leveled) and T2 (extraction space closure) were superimposed and the alveolar bone thickness at root apex level and root length measurements were done.</p><p><b>RESULTS</b>From T1 to T2, the buccal alveolar bone thickness for the upper lateral incisors increased from (1.89±0.83) to (2.47±1.02) mm (P<0.05), and for central incisors and for canines from (2.32±0.71) to (2.68±1.48) mm and from (2.28±1.08) to (2.41±1.40) mm, respectively. According to Sharpe Grading System, the root resorption grade for 69 teeth of 72 was located in Grade 1, two teeth in Grade 2, one tooth in Grade 3.</p><p><b>CONCLUSIONS</b>The improved corticotomy had the potential to increase the buccal alveolar bone thickness and the root resorption in most teeth was in Grade 1 according to Sharpe grading system.</p>
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Humans , Alveolar Process , Diagnostic Imaging , General Surgery , Cone-Beam Computed Tomography , Cuspid , Incisor , Malocclusion, Angle Class III , Diagnostic Imaging , General Surgery , Root Resorption , Pathology , Tooth Root , ZygomaABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of modified corticotomy on periodontal parameters in the treatment of Class III surgical patients facilitated by accelerated osteogenic orthodontics.</p><p><b>METHODS</b>Nine Class III surgical patients at the age of 18-30 (7 females and 2 males) who were systematically and periodontally healthy were involved in the study, including 72 teeth and 216 sites. The modified corticotomy (piezotome) and bone graft (tricalcium phosphate, TCP) in maxillary anterior area were conducted after aligning and leveling the dental arch to facilitate the closing of space in upper dentition. Measurements such as plaque index (PLI), probing depth (PD), bleeding index (BI), recession (REC), keratinized gingiva width (KEG), biotype (BIO) were recorded pre-operation, 1, 2, 4 and 8 weeks post-operation.</p><p><b>RESULTS</b>The differences of PD, BI and REC before and after operation were not statistically significant (P > 0.05). The median value of PLI before and after operation was 0. The percentage of thin biotype and thick biotype teeth was 74% (53/72) and 26% (19/72) pre-operation respectively. The difference of KEG between pre-operation [(5.1 ± 1.4) mm] and 8 weeks post-operation [(5.1 ± 1.2) mm] was not statistically significant (P = 0.658), but the mean value of KEG in other post-operation groups [1 week: (5.7 ± 1.3) mm, 2 weeks: (5.8 ± 1.3) mm, 4 weeks: (5.6 ± 1.4) mm] was significantly higher than those of pre-operation (F = 12.087, P = 0.000).</p><p><b>CONCLUSIONS</b>Modified corticotomy in the treatment of Class III surgical patients facilitated by accelerated osteogenic orthodontics is safe to periodontium.</p>
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Adolescent , Adult , Female , Humans , Male , Young Adult , Dental Plaque Index , Malocclusion, Angle Class III , Maxilla , Osteotomy , Periodontal Ligament , PeriodontiumABSTRACT
BACKGROUND:Nowadays, growth factors are commonly used to induce bone marrow mesenchymal stem cells. However, this is a high-cost method with a great amount of growth factors. In addition, the chondrogenic potential of bone marrow mesenchymal stem cells wil decrease significantly with increasing times of culture. OBJECTIVE:To observe the directed differentiation of bone marrow mesenchymal stem cells co-cultured with human synovial fluid. METHODS:Human bone marrow mesenchymal stem cells were isolated and cultured by adherence screening method. The synovial fluid of the knee was aspirated from healthy volunteers by aseptic operation. Passage 3 human bone marrow mesenchymal stem cells were co-cultured with the fol owing media:synovial fluid+complete medium;synovial fluid+bone marrow mesenchymal stem cells+complete medium;bone marrow mesenchymal stem cells+complete medium. The morphology and growth of the cells were observed under an inverted microscope every day. At days 7, 14 and 21 of induction, toluidine blue staining and immunocytochemical staining were performed. RESULTS AND CONCLUSION:After co-culture with human synovial fluid, human bone marrow mesenchymal stem cells proliferated slowly, and varied from fusiform to oval or polygonal;toluidine blue and col agen II staining were positive. These findings indicate that the synovial fluid has a positive role in the chondrogenic differentiation of bone marrow mesenchymal stem cells. The synovial fluid may contain substances that promote the chondrogenic differentiation of bone marrow mesenchymal stem cells.
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<p><b>OBJECTIVE</b>This study aims to investigate the relationship between the dental and basal arch forms of patients with skeletal class II malocclusion using three-dimensional virtual models and to generate the best-fit curve for these arch forms.</p><p><b>METHODS</b>The mandibular dental casts of 35 skeletal class II patients were taken prior to treatment and were laser scanned. The facial axis (FA) points, which were used to represent the dental arch, and the WALA points, which were used to represent the basal bone, were identified for each tooth from the right first molar to the left first molar. The Pearson correlation coefficients between the intercanine and intermolar widths at the FA and WALA points were determined. Finally, the best-fit curves were generated using the fourth-degree polynomial equation to represent the arch forms.</p><p><b>RESULTS</b>A strong correlation was found between the FA and WALA intermolar widths (r = 0.873). However, a moderate correlation was observed between the FA and WALA intercanine widths (r = 0.534). The difference between the intermolar widths at the FA and WALA points was significant (P < 0.01). No statistically significant difference was found between the intercanine widths (P = 0.481). The radii of curvature of the FA and WALA curves in the anterior teeth area were 15.194 and 20.250, respectively. The regression coefficients of the FA and WALA curves were calculated as R2 = 0.912 and 0.947, respectively.</p><p><b>CONCLUSION</b>For skeletal class II malocclusion patients, the FA and WALA dental intermolar widths showed strong correlation, whereas the FA and WALA intercanine widths exhibited moderate correlation. The FA points were located more labially in the anterior teeth area but more lingually in the posterior teeth area. No statistically significant difference was found between the intercanine FA and WALA widths. The difference between the intermolar widths at the FA and WALA points was significant. The radii of curvature of the FA curve was larger than that of the WALA curve in the anterior teeth area.</p>
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Adolescent , Female , Humans , Male , Cephalometry , Dental Arch , Lasers , Malocclusion , Mandible , Molar , ToothABSTRACT
The purpose of this study was to evaluate the feasibility and clinical outcome of using an oblique AO [Association for the Study of Internal Fixation] metaphyseal locking compression plate [MLCP] in the treatment of adult extra-articular distal humeral diaphyseal fractures via a posterior approach. A series of 19 cases of adult extra-articular distal humeral diaphyseal fractures were surgically treated using an MLCP and lag screws, if necessary, via a posterior approach. All patients were followed for a mean time of 16.6 months [range from 12 to 20 months]. The patients were followed up to 1 year, and radiological assessment was performed to observe fracture reduction and healing. Functional outcome was measured including the range of motion of the shoulder and elbow, by the University of California at Los Angeles and the Mayo Elbow Performance Score. The incidence of iatrogenic radial nerve palsy was 5%. There was no failure of internal fixation and no infection. After 1 year, the University of California at Los Angeles scoring system rated 12 [63.2%] patients as excellent results. The Mayo elbow performance scoring system rated 10 [84%] cases as excellent results. Completely normal alignment was presented in 16 cases. Following surgical treatment of adult extra-articular distal humeral diaphyseal fractures, an oblique MLCP applied via a posterior approach achieved an adequate internal fixation and obtained an excellent functional outcome
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Objective To construct the clinical database on pelvic trauma life cycle so as to provide reference for epidemiological investigations and development of pelvic damage control plan. Methods The existing pelvic trauma data of the hospital information system was analyzed to discuss the main evaluation indicators and data types at different stages, including basic data, injury severity index, underlying disease, fracture classification, specialist treatment, rehabilitation and follow-up information. Results Based on the V3.0 trauma scoring system, the clinical pelvic trauma ACCESS database was developed. The data of 588 patients with pelvic trauma were collected from November 2007 to May 2009 and preliminarily analyzed. Conclusions Clinical database of pelvic trauma can be used as the specific modules of general network trauma database system and a large-scale, multi-center and standard pelvic trauma database may play an important role in preparation of the prospective damage control plans.
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We have investigated the effects of repairing knee osteochondral defects in rabbit by using porous polyamide 66/nano-Hydroxyapatite (PA66/n-HA) combination bone marrow mesenchymal stem cells (MSCs). Eighteen 6-month-old New Zealand rabbits were used to produce the models of 4 mm x 4 mm osteochondral defect in the middle trochlea groove of femur. These models were randomly divided into 3 groups: PA66/n-HA + MSCs Group (Group A), PA66/n-HA group (Group B) and Operation control-group (Group C) in which operation for osteochondral defects was performed but neither material nor cells were implanted. The materials in Group A were seeded with MSCs (5 x 10(5)) in vitro before being implanted in to defects. The materials in groups A and B were 0.5 - 0.8 mm lower than normal cartilage. The animals were killed 1 and 4 months after operation. We assessed the effects by means of macroscopic observation, HE staining, toluidine blue staining, immunohistochemistry assay for type I and type II collagen. Group A displayed a little effect at the 1 month, but at the 4th month, Group A showed better results,compared to Groups B and C. At this time point, the repair tissue of Group A was regular; it presented more metachromatic substance visualized by toluidine blue staining, and it expressed type II collagen(+ +) and type I collagen(+). These results demonstrate that the repair tissue in Group A is nearly hyaline cartilage. So we presume that porous PA66/n-HA provides biomechanical support, and at the same time, MSCs enhance the repair effects.
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Animals , Female , Male , Rabbits , Biocompatible Materials , Bone Substitutes , Hydroxyapatites , Implants, Experimental , Knee Injuries , General Surgery , Therapeutics , Mesenchymal Stem Cell Transplantation , Nanoparticles , Nylons , PorosityABSTRACT
This study was designed to investigate the effects of some Traditional Chinese Medicine (TCM) agents on bone resorption and morphometric features of osteoclasts as well as their relationships. TCM ShengGuZaiZaoSan and XianLingGuBao, were used to treat the experimental fracture. Thirty 6-month-old Chinchilla rabbits were used for the establishment of animal models each with a 3 mm bone defect in the middle of left radius as well as of right radius. These models were divided randomly into 3 groups : ShengGuZaiZaoSan Group (Group A), XianLingGuBao groups (Group B) and control-group (Group C). Every group was further divided into 2 subgroups: a former sacrificed group (14 days after operation) and a latter sacrificed group (31 days after operation). After the rabbits being killed, the samples of their undecalcified calli were subjected to the morphometry study of bone resorption and osteoclasts. Group A had more bone resorption, compared with Group B and C. Both Groups A and B exhibited some changed morphometric features of osteoclasts as compared with Group C (P < 0.05). Simple correlation analysis indicated that bone resorption is mainly correlated with osteoclast numbers, and that in individual group, bone resorption is correlated with osteoclast form factor, area and mean photodensity (P < 0.05). These allow us to conclude that ShengGuZaiZaoSan can increase bone resorption and accelerate bone remodeling by increasing osteoclast numbers at the former stage and can enhance osteoclast function at the latter stage. These changes are beneficial to fracture healing.
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Animals , Female , Male , Rabbits , Bone Remodeling , Bone Resorption , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Fracture Healing , Physiology , Osteoclasts , Pathology , Phytotherapy , Radius Fractures , Drug Therapy , Pathology , Random AllocationABSTRACT
Objective To obtain information on the application value of percutaneous epididymal sperm aspiration(PESA) and percutaneous testicle sperm aspiration(PTSA) in the differentiating diagnosis of obstructive and nonobstructive azoospermia.Methods Sperm recovery procedures were done in infertile men with obstructive azoospermia(OA)(n=37) and nonobstructive azoospermia(NOA)(n=28) by PESA or PTSA.Cytological smears were analysed.Results Sperm was found in the 32 epididymides and 5 testicles of OA group and in the 7 epididymides and 11 testicles of NOA group.Sperm counts were significantly different in two groups.Conclusion PESA and PTSA are efficient methods in differentiating OA and NOA.
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Objective To review the treatment results of intracytoplasmic injection(ICSI) of epididymal or testicular sperm obtained from 38 obstructive azoospermic patients.Methods Sperm was retrieved by percutaneous epididymal sperm aspiration(PESA) or testicular sperm extraction(TESE).Intracytoplasmic injection was performed.The rates of fertilization and clinical pregnancy were evaluated.Control group was set up in which intracytoplasmic injection was performed using sperm of ejaculation.Results Forty-one treatment cycles were performed in the 38 obstructive azoospermc patients.The rates of fertilization and clinical pregnancy were 73.3% and 53.6%.Thirty-three treatment cycles were done in the 31 ejaculatory ones.The rates of fertilization and clinical pregnancy were 75.1% and 48.4%.No significant difference was seen between the two groups.In the obstructive azoospermia group,22 clinical pregnancies were achieved including 13 live deliveries and 3 ongoing pregnancies and 6 miscarriages.In the ejaculatory group,16 clinical pregnancies were achieved including 10 live deliveries and 5 ongoing pregnancies and 1 miscarriages.Conclusions ICSI with PESA or TESE is an effective method for treatment of obstructive azoospermic patients.
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Objective To explore the effective therapies of non-small cell lung cancer(NSCLC) of advanced stage.Method.Two hundred twenty nine patients with NSCLC at advanced stage were treated by three methods including comprehensive therapy,chemotherapy and radiotherapy.Curative effects of various therapy methods were analyzed.Result The total effective rates of the patients who received comprehensive therapy were obviously higher than chemotherapy and radiotherapy alone groups,they were 68 96%,49 17%,50 00%(P
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Objective To explore the effective therapies of no n-small cell lung cancer(NSC LC) of advanced stage.Method.Two hundred twenty nine patient s with NSCLC at advanced stage were treated by thr ee methods including comprehensive therapy,chemotherapy and radiotherapy.Cur ativ e effects of various therapy methods were analyzed.Result The to tal effective rates o f the patients who received comprehensive therapy were obviously higher than che motherapy and radiotherapy alone groups,they were 68.96%,49.17%,50.00%(P ＜0.01) respectively.Conclusion The preference method was c omprehensive therapy.A clos e relationship was found among the total efficacy and pathological classificat ion and clinical staging.