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Background: TAM receptors (Tyro3, Axl and Mertk), as subfamily of receptor tyrosine kinases, are intracellular negative feedback regulators and play a crucial role in regulating inflammation and immune response. Aims: To study the expressions and clinical value of TAM receptors in serum and intestinal mucosa of patients with ulcerative colitis (UC). Methods: Forty⁃five patients who were initially diagnosed as active UC from June, 2020 to May, 2021 at the First Affiliated Hospital of Kunming Medical University were enrolled prospectively. Fifteen healthy subjects were served as the control group. Eight cases each in moderate UC group and healthy control group were selected randomly for detection of TAM receptors in serum and intestinal mucosa by ELISA, real⁃time PCR and Western blotting. The correlations of serum Tyro3 with routine clinical indicators of UC were analyzed by Pearson correlation coefficient. Furthermore, immunohistochemistry was used to detect the expression level of TAM receptors in intestinal mucosa in all UC patients and the healthy controls. Results: Expressions of Axl and Mertk were not fully consistent in serum and intestinal mucosa in UC patients. While the serum Tyro3 level, as well as the intestinal Tyro3 mRNA and protein expressions were consistently increased in moderate UC patients than in controls (all P<0.05). Serum level of Tyro3 was positively correlated with platelet count and C⁃reactive protein, and negatively correlated with albumin in moderate UC patients (r=0.97, r=0.96, r=-0.86, all P<0.05). Positivity rate of Tyro3 in intestinal mucosa of UC patients was positively correlated with the disease severity (all P<0.05). Conclusions: Tyro3 is closely related to UC and positively correlated with the disease severity. It might be a promising novel molecular marker and therapeutic target of UC.
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Background: Endoscopic ultrasonography (EUS) can display the different layers and structures of the intestinal wall, however, its value in diagnosis and assessment of disease activity of inflammatory bowel disease (IBD) is still in exploration. Aims: To investigate the value of colonoscopy and EUS in diagnosis and evaluation of disease severity of IBD. Methods: A retrospective study was conducted in patients with active IBD admitted at the First Affiliated Hospital of Kunming Medical University from September 2017 to September 2019. The results and characteristics of colonoscopy and EUS were analyzed. Results: One hundred and five active IBD patients were enrolled, including 68 cases of ulcerative colitis (UC) and 37 cases of Crohn's disease (CD). Spearman correlation coefficient analysis revealed that the endoscopic score of UC (ulcerative colitis endoscopic index of severity, UCEIS) and CD (simple endoscopic score for Crohn's disease, SES-CD) were both positively correlated with the disease severity (r
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Objective To compare the clinical efficacy of endoscopic ligation between using intensive ligation and non-intensive ligation methods for severe esophageal varices.Methods A total of 89 patients with cirrhosis and esophageal varices in our hospital from 2007 to 2013 were selected and divided into two groups.Patients in the observation group were treated with endoscopic ligation using intensive ligation (39 cases),and patients in the control group were treated with endoscopic ligation using non-intensive ligation (50 cases).Sixshooter multi-band ligator was used for endoscopic therapy.If the number of loop ligature collar was larger than 6 and less than or equal to 12,it was regarded as intensive ligation;if the number of loop ligature collar was smaller or equal to 6,it was regarded as non-intensive ligation.The clinical efficacy was compared between the two groups.Results The average number of ligation in the observation group was greater than that in the control group,there was statistically significant difference (t=29.11,P=0.000).The cure rate at the first time in the observation group was higher than that in the control group,there was statistically significant difference (x2=5.002,P=0.025).No statistically significant difference was found in efficient rate at the first ligation (x2 =2.268,P=0.132).The total effective rates in the observation group half a year,one year,two years and three years after operation was significantly higher than those in the control group (P＜0.05).Conclusion Intensive ligation is a safe,reliable and effective approach for esophageal varices,which could have a high effective rate in the treatment of esophageal varices and reduce the average number of ligation,patients recover well after surgery and have a good long-term prognosis.
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Objective To investigate the expression of HSF2 in colonic mucosa of patients with ulcerative colitis (UC),Crohn's disease (CD),intestinal tuberculosis (ITB),intestinal lymphoma (IL),infectious enteritis,Behcet's disease and normal control.Methods Intestinal tissue samples were retrieved from 2003-2011 archived specimen at the Department of Pathology,and assigned to UC group (n =38),CD group (n =29),ITB group (n =31),IL group (n =32),infectious enteritis group (n =32) and Behcet's disease group (n =28).10 cases were recruited as normal control group.The expression of HSF2 in colonic mucosa were detected by immunohistochemistry.Positive cells were counted by Image Analysis.Results The expression rate of HSF2 in intestinal mucosa of UC patients (64.64 ± 15.17) was significantly higher than that of CD (32.44 ± 5.94),ITB (36.93 ± 6.32),IL (36.16 ± 6.55),infectious enteritis (37.86 ±7.76),Behcet's disease (34.90 ±5.92) and normal control (35.54 ±6.76) (P ＜0.05),while there was no significant difference among the latter six groups (P ＞ 0.05).Conclusion HSF2 is closely related with UC,and may play an important role in the pathogenesis,diagnosis and differential diagnosis of UC.