ABSTRACT
Objective:To prepare human decellular adipose tissue matrix (DAT) as injectable homogenate by a specially developed high-speed soft tissue homogenizer with controllable temperature, and to investigate its cellular compatibility and filling effect.Methods:From March 2019 to March 2021, DAT was obtained in the 940th Hospital from normal adipose tissue after decellular treatment. The DAT was mixed with normal saline at the rate of 1: 12. The specially developed high-speed soft tissue homogenizer with controllable temperature was used to conduct homogenization at 803×g for 10 min. The produced DAT homogenizer could pass through the 27G needle smoothly. DAT homogenate was observed under scanning electron microscope and its cytocompatibility was detected. Finally, granular fat, DAT homogenate and DAT homogenate + ADSCs were injected into the back of rats respectively, and the filling effect, angiogenesis ability and inflammatory infiltration were compared.Results:After decellular treatment, adipose tissue changed into DAT without cellular residue. The particle size of DAT homogenate was about (749.91±136.79) nm, which was prepared by the specially developed temperature controlled high-speed soft tissue homogenater. The adhesion rate was (92.16±1.00) %, and the A value increased with time. The cell growth was good, and the homogenate had no toxicity to the cells. In vivo experiment, the filling effect of DAT homogenate and DAT homogenate + ADSCs was significantly better than that of granulated fat group ( P<0.01). In the granulated fat group, a large number of adipocytes were necrotic and fused to form oil sacs, while DAT homogenate, DAT homogenate + ADSCs showed no obvious degradation, and some adipocytes were generated. The results of CD31 staining indicated that the number of microvessels in DAT homogenate group and DAT homogenate + ADSCs group was higher than that in granulated fat group ( P<0.01). The results of CD68 staining indicated that the inflammatory infiltration in DAT homogenate group and DAT homogenate + ADSCs group was lower than that in granule fat group ( P<0.01). Conclusions:Using the self-developed temperature controlled high-speed soft tissue homogenate device, DAT could be prepared into DAT homogenate that can pass through 27G needle. It has good biocompatibility and filling advantages, and injective process is simple, and the trauma is small, and so it could be used as filling material.
ABSTRACT
Objective:To identify the influence of age factors on biological characteristics of human adipose-derived stem cells (ADSCs), and to compare the differences in morphology, aging characteristics, differentiation potential, surface marker expression, and proliferation ability of human ADSCs with different ages and the effect of human ADSCs of different ages on proliferation and migration of fibroblasts.Methods:Adipose tissues from 27 individuals of healthy beauty treatments, aged 0-59 years, were collected from Department of Burns and Plastic Surgery, the 940th Hospital of Joint Logistics Support Force from January 2020 to December 2020. According to the age of the donor, they were divided into 6 groups. 0-9 years old group (4 cases), 10-19 years old group (4 cases), 20-29 years old group (5 cases), 30- 39 years old group (5 cases), 40-49 years old group (5 cases), and 50-59 years old group (4 cases). We obtained and cultured the ADSCs of each group, subcultured to obtain the ADSCs, and observed the morphological characteristics of each group; the proliferation ability of each group of ADSCs was detected by CCK-8 method; the differentiation potential of each group of ADSCs was detected by in vitro adipogenesis and osteogenic induction. Flow cytometry was used to detect the surface marker expression levels of each group of ADSCs, and qPCR was used to detect the expression levels of HGF, PPAR-γ, type Ⅲ collagen and DbX2 in each group. We also collected the skin tissues of a healthy person from Department of Burns and Plastic Surgery of the 940th Hospital of Joint Logistics Support Force in 2020, obtained and cultured fibroblasts, subcultured to obtain the second generation of fibroblasts, and prepared each group of ADSCs conditioned medium (ADSCs-CM); the proliferation ability of fibroblasts cultured in each group of ADSCs-CM was detected by CCK-8 method, and the migration ability of fibroblasts cultured in each group of ADSCs-CM was detected by cell scratch method. SPSS 21.0 software was used for data analysis and P<0.05 was considered statistically significant. Results:The cells of all age groups were in a typical spindle shape. The cells of the younger age group showed small nuclei and the cell morphology were uniform and similar; the cells of the older age group had larger nuclei and uneven morphology. With aging, the proliferation ability of ADSCs gradually decreased. In vitro adipogenic differentiation induction results showed that with the increase in age, the ability of ADSCs to differentiate into adipocytes gradually decreased. In vitro osteogenic differentiation induction results also demonstrated that with the increase in age, the ability of ADSCs to differentiate into osteoblasts gradually decreased. The expression rates of positive surface markers of ADSCs in each group of ADSCs were all above 93%. With aging, the expression levels of PPAR-γ, HGF, and type Ⅲ collagen decreased, and the expression levels of DbX2 showed an upward trend with age. The number of β-galactosidase staining positive cells increased with age, while the difference between groups A, B and C was not statistically significant, and the difference between groups A and D, E, and F was statistically significant ( P<0.05). The ADSCs conditioned medium of each group was able to promote the proliferation of fibroblasts, but the difference between the groups was not statistically significant. With aging, the ability of ADSCs conditioned medium to promote the migration of fibroblasts gradually decreased. Conclusions:The proliferation ability and multidirectional differentiation ability of human ADSCs and the ability to secrete a variety of cytokines that promote the proliferation and migration of fibroblasts show a downward trend, but the adipogenic differentiation ability of ADSCs declines significantly after the age of 40 years. ADSCs in all age groups could promote the proliferation and migration of fibroblasts.
ABSTRACT
Objective:To study the characteristic of P300 elicited by the Oddball pattern among the depressed patients.Methods:Twelve untreated young depressed patients and sixteen normal people as a control group participated.Auditory P300 and novel P300 were induced and recorded under the Oddball pattern.Results:The depressed patients’ reaction time(755?244ms) to the target stimuli was significantly longer than that of the control group(386?114ms).As compared with the normal,the depressed patients’ amplitudes of P3b in the parietal(P
ABSTRACT
Objective: To investigate validities of MMPI- 2 measures of general maladjustment in people who had expe- rienced traumatic events. Methods: Participants were 43 patients with stress disorder (SD) and 44 well- adjusted trauma survivors(TS). Validities of 7 MMPI- 2 measures of general maladjustment were compared with the mean scores on 8 clini- cal scales (M8), which were considered as the criterion measure of general maladjustment. Results: Patients with SD ob- tained significantly higher scores on 6 measures and M8 than TS. Most of the measures were significantly related to M8 in both groups. Although F and PK sometimes added significantly to the variance accounting for the criterion measure, Pt added significantly and meaningfully to each of the other MMPI- 2 measure in predicting maladjustment. Conclusion: The results support the use of the MMPI- 2 as a measure of general maladjustment in trauma survivors. Pt emerges consistently as a good indicator of general maladjustment.
ABSTRACT
Objective: To investigate psychopathology and diagnostic efficiency with MMPI- PK scale for patients with stress disorder(SD). Methods: MMPI- 2s were administered to forty- three in patients with SD and forty- four well- adjusted trauma survivors as controls. Results: Patients with SD obtained significantly higher PK scores than controls. Gender, types of traumatic events and types of diagnoses for patients with SD did not substantially influence PK scores. When an optimal cut- off point of 17 was utilized, sensitivity and specificity obtained by PK assessment are respectively 94.9% and 66.7%. Conclusion: Patients with SD and trauma survivors show significantly different psychopathology. PK scale was sen- sitive to the assessment of posttraumatic stress symptoms though it produced more false- positive than false- negative errors.
ABSTRACT
Objective: To investigate the relationship between the cognitive appraisal,coping style and the mental health of military personnel. Methods: 370 military personnel were assessed with Life Event Inventory for Compulsory Servicemen of Land Army(LEI-CSLA), Social Support Scale, General Perceived Self-Efficacy Scale(GSES), Simplified Coping Style Questionnaire and Symptom Checklist 90(SCL-90). Results: The results showed that the recruits and the old soldiers both had good mental health levels. Compared with the old soldiers, the recruits’ were better, and had significant difference in life events and objective social support. Life events and negative coping style had significant negative correlations with psychological health(r=-0.543, r=-0.382, P
ABSTRACT
Objective: To observe the effects of exposure to white noise on the procession of learning and memory, and the expression of NOS (nitric oxide synthase) in hippocampus of rats Method: 20 rats were randomly divided into two groups for observation of spatial learning and memory, one group exposed to 80 dB white noise during Y-maze training, the other group received Y-maze training under normal condition Another 24 rats were randomly divided into 3 groups, they experienced normal Y-maze training, noise Y-maze training and sole noise as control respectively Immunohistochemical assay was used to investigate the expression of NOS positive neurons Result: Noise hampered the ability of rats' spatial learning and memory, furthermore, the number of NOS positive neurons in hippocampus of noise training group was less than that of normal training group Conclusion: The activity of NOS positive neuron in hippocampus was decreased by exposure to noise, suggesting the inhibition of learning dependent long term procession in hippocampus and corresponding difficulty in obtaining and maintaining of memory
ABSTRACT
Objective: To investigate psychopathology and diagnostic efficiency with MMPI-2 in patients with stress disorder (SD). Methods: MMPI-2s were administered to twenty-nine patients with acute stress disorder (ASD), fourteen patients with posttraumatic stress disorder (PTSD) and forty-four well-adjusted trauma survivors. Results: Patients with ASD obtained significantly higher scores on F, Fb, Fp, Pa, Pt, Sc and Ma and lower on K, S than controls. PTSD patients scored significantly higher on F, Fb, Fp, Hs, D, Hy, Pa, Pt and Sc and lower on S than controls. There were significant differences in scores of Hs, D and Hy between patients with ASD and with PTSD. M678 code type was found as the mean profile for SD patients. Discriminate analysis indicated that Pa and Sc were the most important variables and it successfully classified 83.9% and 72.0% respectively in training sample and cross-validation sample. Conclusion: Patients with SD and trauma survivors show significantly different psychopathology and there were also differences between patients with ASD and with PTSD. MMPI-2 scales especially Pa and Sc were sensitive to posttraumatic stress symptoms.