ABSTRACT
Objective To establish mouse model of type 2 diabetes peripheral neuropathy and measure its intra-epidermal nerve fiber density (IENFD).Methods Male C57BL6 mouse were ran-domly divided into four groups:group HS (n =6):high-fat diet+single streptozotocin intraperitoneal injection (120 mg/kg);group H (n =6):high fat diet+buffer injection;group S (n =6):standard chow diet+single streptozotocin intraperitoneal injection (120 mg/kg);group C (n = 6 ):standard chow diet+buffer injection.The 24th week was the end point of the experiment,and random glucose, homeostasis model assessment of insulin resistance (HOMA-IR),mechanical threshold,and IENFD were measured.Results Group HS had significantly higher random glucose and HOMA-IR than other groups (P <0.01),had significantly lower mechanical threshold than other groups (P <0.05), had significantly lower IENFD than groups S and C at 24th week (P <0.05 );group H had signifi-cantly higher random glucose and HOMA-IR than group C at 24th week (P <0.01),had no signifi-cant difference in mechanical threshold compared with group S and group C,and had no significant difference in IENFD compared with group HS.Conclusion A mouse model of type 2 diabetes periph-eral neuropathy was successfully established,and the IENFD was found to be decreased significantly.