ABSTRACT
OBJECTIVE:To screen the α-glucosidase inhibitory active part from Pothos chinensis. METHODS:The aqueous extractions of P. chinensis were extracted by petroleum,ethyl acetate,n-butyl alcohol in turn to obtain different polarparts. Effect of each part on α-glucosidase inhibitory activity was determined,and enzyme inhibition kinetics was conducted for the screened parts with strong activity and relatively high yield rate;effects of each part on blood glucose level of mice loaded with glucose,su-crose and starch were respectively determined (using Acacoside tablet as positive control). RESULTS:Enzyme inhibition kinetics in vitro showed the ethyl acetate part [yield rate was 0.40%,enzyme activity inhibition rate was(72.90±2.85)%] had strongα-glu-cosidase inhibitory activity and showed a dose-dependent,fast,non-competitive and reversible model. Results of in vivo glucose tol-erance indicated that Acacoside tablet and each part of P. chinensis had no effects on blood glucose level of glucose-loaded mice (P>0.05);while Acacoside tablet and ethyl acetate part in P. chinensis could reduce 30,60 min blood glucose level of su-crose-loaded mice and 30,60,120 min blood glucose level of starch-loaded mice(P<0.05 or P<0.01). CONCLUSIONS:Ethyl acetate part is theα-glucosidase inhibitory active part from Yao medicine P. chinensis.
ABSTRACT
After the monoclonal antibody drug come out,it has played an important role in the clinical treatment,especially in tumor therapy.With the further understanding of monoclonal antibody,it has been applicated in the food, transgenic animal and plants,plant virology aspect. However,rare research of natural medicine have been reported in China.The monoclonal antibody-based enzyme-linked immunosorbent assay method, which is sensitivity,accuracy,fastness and simpler,can be used in quantitative analysis of bioactive compound in the natural products. It also can be used in isolation and purify effective component of the natural medicine, the pharmacokintic study,the quanlity control, screening candidacate drug and expanding the resources of medicinal plant It is important to exploit and utilizate the natural medicine,and promote the progess of modern chinese medicine.