ABSTRACT
AIM: To investigate the relationship between genotypes of rs628031, rs650284, rs683369 of SLC22A1 gene and the toxicities and clinical response of oxaliplatin in patients with colorectal cancer. METHODS: A total of 72 patients diagnosed as colorectal cancer during January 2018 to June 2018 were selected and all patients received oxaliplatin treatment. Their peripheral venous blood was collected and genotyping was conducted by using SNaPshot. The toxicities including gastrointestinal toxicity, hematological toxicity and peripheral neurotoxicity were evaluated according to the Common Terminology Criteria Adverse Events (CTCAE) Version 5.0. Clinical response was evaluated according to the Response Evaluation Criteria in Solid Tumors (RECIST Version 1.1). RESULTS: The results of Chi-test showed that different genotypes of SLC22A1 SNP sites rs628031 and rs683369 may be related to the toxicities and clinical response of oxaliplatin significantly. Specifically, when compared with the patients with GG type of rs628031, the patients with the GA or AA type had a lower incidence of grade 3 nausea and vomiting (P=0.017) and may also be less responsive to efficacy (P=0.008). When compared with the patients with CC type of rs683369, the patients with the GC or GG type had a lower incidence of grade 3 nausea and vomiting (P=0.002) and may also be less responsive to efficacy (P=0.014).CONCLUSION: The polymorphisms of SLC22A1 gene are closely related to the toxicities and clinical response of oxaliplatin in patients with colorectal cancer, which may be helpful for improving clinical treatment.
ABSTRACT
Background Despite the growing occupational stress of medical personnel, there is no occupational stress risk management index system for medical personnel in China, and the related risk management work is unfounded. Objective To construct a work-related stress risk management index system for medical personnel in China based on Delphi method. Methods On the basis of literature review and expert interview, an index pool of work-related stress risk management for medical personnel was preliminarily constructed. Through two-round Delphi method, experts' opinions on the importance, operability, familiarity, and judgment basis of candidate indicators were collected, and an index system for work-related stress risk management of medical personnel was constructed. Results Fifteen expert opinions were collected in each of the two rounds, with a recovery rate of 100%. The expert authority coefficients were all >0.9. In the first round, the importance score was 9.78±0.35, the coefficient of variation was 0-0.22, and the Kendall coordination coefficient was 0.215 (P<0.01); in the second round, the importance score was 9.82±0.26, the coefficient of variation was 0-0.13, and the Kendall coordination coefficient was 0.208 (P<0.01). Finally, the index system of work-related stress risk management for medical personnel was composed of 10 first-level indexes, 28 second-level indexes, and 47 third-level indexes. The 10 first-level indexes were job demand, job control, organizational support, interpersonal relationships, role conflict, doctor-patient conflict and medical disputes, work-life conflict, professional self-identity, workplace environment, and social environment, respectively. Conclusion A preliminary refined work-related stress risk management index system for medical personnel in China has been established, but the indicators will be further modified by verification and application of the indicator system.
ABSTRACT
Norovirus, as one of the main pathogens causing non-bacterial gastroenteritis, can cause serious public health problems and economic losses around the world. In recent years, the outbreaks caused by the virus in China are on the rise. Human Norovirus (HuNoV) can hardly be cultivated in-vitro. The nucleic acid detection method (such as RT-qPCR) has the highest sensitivity and specificity, but it was not established that the correlation between the detected viral genome and viral infectivity, which leads to inaccurate judgment of safety risks. Here, the in-vitro and in-vivo culture models, viral genome integrity and capsid protein integrity were cut into three aspects. The research progress and characteristics of infectious Norovirus identification technology in recent years were reviewed and discussed, and the future development trend of this technology was prospected. The aim is to further improve the accuracy of Norovirus quantitative detection and provide a theoretical basis for its application in the field of food safety testing.