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Aim To investigate the effects of cimifugin on mouse atopic dermatitis (AD) induced by fluorescein isothiocyanate (FITC) and further explore the mechanism of its action. Methods ICR mice were randomly divided into blank group, model group, positive group (dexamethasone),low dose group,high dose group and administration group of cimifugin. FITC solution was applied to the shaved abdomen of mice in the sensitization stage, and 0.6 % FITC solution was applied to attack the ears of mice in the stimulation stage. The administration groups were given medicine for seven consecutive days. The effects of cimifugin on body weight, thymus index and spleen index of mice were detected. Ear inflammatory cell infiltration was observed by HE staining. The ear swelling of mice was measured, and Th2 cytokines IL-5,IL-13 and the key promoter of allergy IL-33 were detected by ELISA. The epithelial barrier structural proteins, filaggrin, claudinl,occludin and E-cadherin,were detected by immunohistochemistry and Western blot. Results Compared with the blank group, the model group showed significant AD symptoms. Compared with the model group, cimifugin transdermal administration group significantly reduced ear inflammatory cell infiltration,ear swelling, IL-5,IL-13 and IL-33, and significantly increased the expression of filaggrin and occludin. Conclusions Transdermal administration of cimifugin could significantly inhibit AD in mice, and its mechanism involves repairing epithelial barrier function, restoring filaggrin and occludin, inhibiting allergy promoting factor IL-33, and finally inhibiting AD inflammation.
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Nodular goiter has become increasingly prevalent in recent years. Clinically, there has been a burgeoning interest in nodular goiter due to the risk of progression to thyroid cancer. This review aims to provide a comprehensive summary of the mechanisms underlying the therapeutic effect of Chinese medicine (CM) in nodular goiter. Articles were systematically retrieved from databases, including PubMed, Web of Science and China National Knowledge Infrastructure. New evidence showed that CM exhibited multi-pathway and multi-target characteristics in the treatment of nodular goiter, involving hypothalamus-pituitary-thyroid axis, oxidative stress, blood rheology, cell proliferation, apoptosis, and autophagy, especially inhibition of cell proliferation and promotion of cell apoptosis, involving multiple signal pathways and a variety of cytokines. This review provides a scientific basis for the therapeutic use of CM against nodular goiter. Nonetheless, future studies are warranted to identify more regulatory genes and pathways to provide new approaches for the treatment of nodular goiter.
Subject(s)
Humans , Goiter, Nodular/metabolism , Medicine, Chinese Traditional , Thyroid Neoplasms , Apoptosis , ChinaABSTRACT
Objective:To observe the long-term behavioral changes in movement, emotion, and learning and memory of newborn mice with hypoxic ischemic brain damage (HIBD). Methods:A total of 50 ten-day old newborn C57BL/6 mice were divided into control group (n = 20) and HIBD group (n = 30). The left common carotid artery was ligated in HIBD group and stayed in anoxic chamber for 45 minutes. All the mice were tested with suspension test, light/dark box test, elevated plus maze test, object recognition test and Y maze test two months after surgery. Results:There were 19 mice modeled successfully. Compared with the control group, the suspension test scores decreased (t = 2.785, P < 0.05); the time of latency of light/dark box test increased (t = -4.320, P < 0.001), the time and frequency in light box decreased (t > 2.603, P < 0.05); the time in open arm decreased (t = 4.576, P < 0.001) and the time in close arm increased (t = -3.287, P < 0.01) for the elevated plus maze test; the time nearing old object increased (t = -2.116, P < 0.05) and object recognition index decreased (t = 2.823, P < 0.05) for object recognition test; the time in the initial and novel arms decreased (t > 2.191, P < 0.05) for Y maze test in HIBD group. Conclusion:The long-term disorders of behavior may include disabilities of motor, learning and memory, and disorder of anxiety, in newborn mice with HIBD.
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Aim To investigate the effect of Radices Paeoniae Alba extract on estrogen receptor (3 ( ERp ) and serotonin transporter(SERT) in the hippocampus of rat model of premenstrual syndrome( PMS) liver-qi inverse syndrome. Methods The rats were randomly divided into the normal group, the model group, the Radices Paeoniae Alba extract group, the fluoxetine group and the ER(3 agonist group by the vaginal smear microscopy and the open field score. The model group rats were prepared by electrical stimulation to prepare PMS liver-qi inverse syndrome. After five days of administration, the hippocampal tissues of each group were separated. ERp and SERT mRNA and protein expression in each group were determined by RT-PCR and immunofluorescence histochemical method. Results During the estrous reception period, there was no significant difference in the behavior score of the open- field test and the expression of ERp and SERT mRNA and protein in the hippocampus of each group. Estrous non-reception period; compared with normal rats, the total distance of open field test in model rats increased significantly. The expression of ER(3 mRNA in hippocampus significantly decreased and SERT mRNA significantly increased. In model group, the pyramidal cells in hippocampal CA1 and CA3 brain areas of rats were irregularly arranged, and the number of ERp positive cells and protein expression decreased. The number of SERT positive cells and protein expression were significantly up-regulated. After the Radices Paeoniae Alba extract was given, the above indexes were significantly improved, and no significant difference was found between them and fluoxetine group and ER agitator group. Conclusions Radices Paeoniae Alba extract may adjust the PMS card of diseases with inverse nega-tive emotions possibly by improving the hippocampus ER|3 and SERT mRNA and protein expression.
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There is a high incidence of Premenstrual syndrome in childbearing aged women. Its clinical symptoms including affect seriously many aspects of physical and mental health disorder of women, resulting in interpersonal relationship deteriorious, decreased quality of life, and even suicidal tendency. The biochemical mechanism of the disease is complex, but the social psychological factors such as personality and habits have reached a consensus on the induction of the disease, which will be the next research direction. Further research will reveal the pathogenesis of PMS from another angle. Based on the field of psychology, the article reviews the relationship between social psychological factors such as personality, personality and coping style and the occurrence and development of PMS, and the role of psychological interventions in regulating diseases.