ABSTRACT
OBJECTIVE@#To detect the levels of Epstein-Barr virus (EBV) latent membrane protein 1 Antibodies (LMP1-Ab) in nasopharyngeal carcinoma (NPC)sera and discuss the clinical significance of this test in diagnosis, prognosis, and immune-targeted therapy of NPC.@*METHOD@#Enzyme-linked immunosorbent assay (ELISA) and Western blot method were used to detect the LMP1-Ab levels in 61 NPC sera, 30 nasopharyngitis sera, and 55 normal sera. The relationship between the LMP1-Ab level and clinical and pathological features of NPC was analyzed.@*RESULT@#ELISA test showed that LMP1 antibodies level was significantly higher in nasopharyngeal carcinoma group than those in nasopharyngitis group and in healthy group and there were statistical significances (all P0.05). Western blot test revealed that the expression of LMP1 antibodies was higher in NPC sera than in nasopharyngitis sera and in normal sera.@*CONCLUSION@#LMP1-Ab level was higher in nasopharyngeal carcinoma group than in nasopharyngitis group and in normal group. Therefore, LMP1 may be considered as a tumor correlated antigen to help the diagnosis and immune-targeted therapy of NPC.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Antibodies, Viral , Blood , Carcinoma , Case-Control Studies , Enzyme-Linked Immunosorbent Assay , Herpesvirus 4, Human , Allergy and Immunology , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms , Blood , Pathology , Viral Matrix Proteins , Allergy and ImmunologyABSTRACT
Objective To investigate the characteristics of acoustic parameters and spectrograph of organic and functional voice disorder and evaluate the value of acoustic analysis technique by computer in the differential diagnosis of voice disorder. MethodsThe voice signals of sustained vowel "a" were measured with a microcomputer (Dr.Speech Software) in 70 healthy adults, 60 patients with varying organic diseases of vocal cords and 50 cases with functional voice disorder. The acoustic parame ters (jitter, shimmer, NNE) and spectrographic characteristics were analyzed and compared. ResultsIn 60 patients with organic diseases of vocal cords, all the acoustic parameters were significantly increased and the spectrograph showed irregularity,breakage or lack of harmonic waves and formants, and increase of noise. In 50 cases with functional voice disorder, the shimmer and NNE were significantly increased but the jitter was usually normal. The spectrograph showed pathologic changes of harmonic waves and formants in middle and high frequencies. The normal acoustic characteristics could be found in 94% (47/50) of the patients with functional voice disorder. ConcluisionThe acoustic analysis by computer technique is of value in the differential diagnosis of or ganic and functional voice disorder.
ABSTRACT
Objective To investigate the acoustic f eatures of voice in patients with abnormal lung function, evaluate the effect o f lung dysfunction on acoustics of voice,and observe the relationship between re spiration and phonation. Methods The voice signals of sustained vowel /a/ were mea sured by a micro-computer with Dr.speech science for windows software in 50 pati ents with abnormal lung function and 50 healthy adults. The acoustic parameters( F0,jitter,shimmer,intensity,NNE) were collected and analysed in lowest pitch,no rmal pitch and highest pitch.All patients were divided into two groups according to sex. Results In patients with abnormal lung function ,the pa rameters (shimmer, jitter, NNE,)were significantly increased or others(F0,inten sity) were significantly decreased in lowest pitch. Intensity decreased si gnificantly in highest pitch . There was a positive relationship between the de grees of lung dysfunction and the parameters of acoustics. Conclusion Abnormal lung function affected significantly on acoustics of voice in three different pitches; The changes of the parameters were related to the degrees of lung function.The results showed that respiratory activities play an important role in phonation.