Determination of Plasma Concentration in Rats after Intravenous Injection Cinnarizine by HPLC-FLU / 中国药房

OBJECTIVE:To develop an HPLC-FLU method for the determination of cinnarizine in rat plasma and to study its pharmacokinetics. METHODS:6 rats were given cinnarizine injection(3 mg?kg-1)by venous cannula and after 5 min,10 min, 20 min,40 min,60 min,120 min and 240 min,blood samples were collected. HPLC-FLU was established to determine the plasma concentration of cinnarizine and the pharmacokinetic evaluation was carried out. RESULTS:The linear range of cinnarizine was 10～1 000 ng?mL-1(r=0.999 6)and the limit of detection of cinnarizine was 0.2 ng?mL-1,the average recovery being 95.9%～98.6% with RSD