ABSTRACT
Objective To investigate the relationship of body mass index (BMI), blood glucose, and blood calcium with se-verity of acute pancreatitis .Methods A total of 127 cases of patients with acute pancreatitis was divided into 10 cases of severe acute pancreatitis group (SAP group), and 52 cases of mild acute pancreatitis group (MAP group), according to the severity.BMI, levels of blood sugar and blood calcium of two groups were compared , respectively .Correlation analysis was carried out among those parame-ters.Results The differences in BMI, blood sugar, and blood calcium between SAP and MAP groups were statistically significant , respectively ( P <0.05).The differences in BMI, blood sugar, and blood calcium between patients [Ranson score≥3, Balthazar computed tomography(CT) grade D or E, and computed tomography severity index (CTSI)≥3)] and patients [Ranson score<3, Balthazar CT grade A , B, or C, and CTSI<3 ( P <0.05 ) ] were statistically significant ( P <0.05 ) , respectively .Pearson correla-tion coefficient analysis showed significantly positive correlations among BMI , blood glucose , blood calcium , Ranson score , Balthazar CT grade, and CTSI ( P <0.05).Conclusions Severity of acute pancreatitis patients was related to BMI , blood sugar, and blood calcium.All of them can be used as evaluation index of disease progression and prognosis in patients .
ABSTRACT
AIM: In order to investigate the possible cumulative and chronic toxicity of paralytic shellfish poisoning (PSP) and provide more information on toxicity of PSP. METHODS: The sub-acute toxicity of PSP was evaluated in the rat. PSP was extracted from the seafood in market, and the toxicity of the extract was determined by mouse bioassay. The extracts in different toxicity were administrated to rats through gastrotube for 35 days. The biochemical and pathological changes in vital tissues in rats were examined by the detections of some function indexes in blood and urine and the observations under optical microscope during both the exposure period and the subsequent 10-day withdrawal term. RESULTS: No biochemical and pathological changes in tissues occurred for the control and low-dose group (4.6 ?g STX/kg), whereas some changes happened for the middle (9.2 ?g STX/kg) and high groups (18.4 ?g STX/kg). In experiment, some renal function indexes changed in the mid-dose group, and some of the cardiac, hepatic and renal functions indexes altered for the high dose group with some changes in weight of the thymus gland and spleen. What is more, the liver and kidney became indistinct with some inflammatory changes, and some muscles had ruptured for the 40% rats in high-dose group. CONCLUSION: These results suggest that long-term intake of PSP at a concentration, which doesn't exceed the threshold of standard, has some toxicity on rats, and that the toxicity of PSP has an accumulative effect. In a word, it is unsafe for us to eat frequently bivalve seafood polluted by PSP toxins.
ABSTRACT
ve To study the growth curve of Alexandrium tamarense and the toxicity of paralytic shelfish poisoning(PSP) selected in different growth phases, as well as to quantitatively analyze the toxins and compare the different efficiency of breaking cell wall and their virtues and defects between freezing-thawing method and supersonic method, and to provide theoretical basis for the selection of the methods for extraction of PSP. Methods Cells were collected by suction filter, cell wall was broken by freezing-thawing method and supersonic method, the toxicities of Alexandrium tamarense among different growth phases were detected and compared. Results The growth of Alexandrium tamarense typically showed three phases which included a lag phase(0~9 days), an exponential growth phase(10~18 days), and a stationary phase(19~22 days) . The maximum toxicity of cells in the stationary phase appeared, especially in day 21 when the population of cells reached to 9.43?10-6 MU/cell, although the cells in the exponential phase grew fastest. Toxicity of the cells in the lag phase broken by freezing-thawing method was more stronger than that by supersonic method, that meant the toxicity of cells was lowered after the supersonic treatment. In additional, the filtering method for collecting cells presented a lower recovery rate of average 69.0% . Conclusion As for the comparing of freezing-thawing method and supersonic method, it was found that, when cells were in the same phase, the supersonic method had remarkable advantages, but it also had the disadvantage of lowering the tox-icity of cells. When in the same condition of growth and method of fragmentation, cells in the lag phase were broken most easily, and cells in the stationary phase were more difficult to be broken than those in the other two phases. Toxicity of cells was larger and larger with the time of incubation. The toxicity of cells in the lag phase had reached a higher intensity, although toxicities of cells in the next two phases would increase a little.